邱建智教授 (Charles Chien-Chih Chiu)
關於我
聯絡方式:
- 高醫生技系的研究發展現況報導 [點我]
- 研究室: N1022 (實驗室: N1020), 第一教學大樓 (點我，實驗室語音簡介)
- 電話: +886-7-3121101 ext. 2368 傳真: +886-7-3125339 E-mail: cchiu@kmu.edu.tw
- Researchgate: https://www.researchgate.net/profile/Chien_Chih_Chiu/
- ORCID: http://orcid.org/0000-0001-7307-2468
學經歷:
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中山大學生物系 理學士 (1996) 及生物科學系碩士 (1998) -
臺灣師範大學生命科學系理學博士 (2004) 及博士後研究員 (2004-2005) -
高雄師範大學生物科技學系 兼任助理教授 (2006) -
高雄醫學大學生物科技學系 助理教授 (2005-2011) /副教授 (2011- 2014) / 教授 (2014.09 - Present) -
高雄醫學大學附設醫院 轉譯醫學研究中心 兼任副研究員 (2014) -
高雄醫學大學醫學研究所 合聘教授 (2016.08 - Present) -
中山大學生物科學系 合聘教授 ( 2016.08 - Present) -
高雄醫學大學附設醫院 臨床醫學研究中心 兼任研究員 (2017.08 - Present) - 高雄醫學大學生物科技學系 系主任 (2018.08-2021.07)
- 高雄醫學大學 癌症研究中心成員
行政服務：
| NO | 學年 | 科室 | 職稱 |
| 1 | 107-109 | 生物科技學系 | 系主任 1070801~1100731 |
| 2 | 107 | 生技醫藥產業研發碩士學位學程 | 學程主任 1071201~1080731 |
| 3 | 106 | 生命科學院研發暨國際組 | 組長 1060801~1070731 |
| 4 | 104 | 教務處註冊課務組 | 組長 1040801~1041021 |
| 5 | 103 | 教務處研究生教務組 | 組長 1040310~1040731 |
學術服務：
1. Associate Editor, BMC Cancer, part of springer nature BMC Cancer is a peer-reviewed journal that considers articles on all aspects of cancer research, including the pathophysiology, prevention, diagnosis, and treatment of cancers. The journal welcomes submissions concerning molecular and cellular biology, genetics, epidemiology, and clinical trials. https://bmccancer.biomedcentral.com/
2. Reviewer board, Cancers. Cancers (ISSN 2072-6694; CODEN: CANCCT) is a peer-reviewed Open Access journal of oncology published semimonthly online by MDPI. https://www.mdpi.com/journal/cancers
臨床試驗微學程負責教師 [連結學校學程中心] 本系有相當多畢業生從事臨床試驗專員工作，鼓勵有興趣的同學們申請學程，在校內即預先充實自我，提早強化職場競爭力!!- 微學程介紹及影片 (修滿必要學分將申請微學程證書，並註記所通過之修課科目)- 課程規劃及要求
微學程選修學生之心得 (108學年修課學生回饋) 首先想先感謝高醫校方無償提供系上課程外，如此多元化的學分學程/微學程之課程，讓我們學生能以探索自己系所外專業領域之興趣，可以慢慢了解自己喜歡甚麼，以及對於可能適合從事甚麼領域……等的專業興趣培養。更重要的是，選修了學分學程及學分微學程，讓我們學生在大學經歷四年畢業後，能比同期學生多了更多競爭力。 我選擇選修的學分學程是「臨床試驗微學分學程」，這領域的學分學程課程與系上專業領域不盡相同，讓我自己的課業壓力有強大的挑戰，但也因此拓展開闊不同領域的知識，其中一門公衛系王姿乃教授開授的選修「流行病學」，讓我對此方面專業特別印象深刻，師長講解淺顯易懂，因應武漢肺炎疫情時事，在閱讀新聞或媒體資訊時也能看得懂些專有名詞的意義，相當實用。另外，在生物系陳炤杰教授開授的選修「試驗設計」、「試驗設計實習」也是受益良多，小班制的教學使我們每個學生能在課堂的軟體操作上即時接受老師的指導。
主負責授課科目：基因體學與蛋白質體學腫瘤生物學書報討論/專題研究 (大學三年級與四年級)/實驗室實習 (大學部二年級)
講義下載 (請暫時到 e-learning 區下載)： l 大學部課程普通生物學普通生物學實驗服務學習 電腦生物分子生物學 自由基生物學 基因體及蛋白質體學腫瘤生物學 l 研究所課程腫瘤生物學特論訊息傳遞特論
研究興趣:
- 程序性細胞死亡 (包括細胞凋亡apoptosis以及非凋亡性細胞死亡 non-apoptotic programmed cell death)
- 抗癌藥物篩選與機制探討 (Chemotherapeutics screening and the mechanisms)
- 發展癌細胞致敏化策略 (The strategies of cancer sensitization)
實驗室簡介
- 實驗室語音簡報 (Presented in Mandarin)
- Researchgate: https://www.researchgate.net/profile/Chien_Chih_Chiu/
- ORCID: http://orcid.org/0000-0001-7307-2468
細胞凋亡(apoptosis)是我們最熟悉的程序性細胞死亡。多細胞生物為了發育與維持恆定而啟動程序性細胞死亡機制，特徵包括細胞膜皺縮、細胞內膜磷脂質外翻(PS Flipping)、DNA斷裂及形成凋亡小體並且被周圍細胞或巨噬細胞吸收，而不會產生發炎反應。少數真菌或原生動物也有被報導有細胞凋亡產生，如黏黴菌Dictyostelium discoideum， Trypanosoma cruzi 與 Trypanosoma brucei rhodensiense , 鞭毛菌Tetrahymena。目前癌症病人主要治療方式之一為小分子藥物為基礎的化學治療，並且過去大部分的抗癌藥物設計原理主要著重於誘導細胞凋亡，希望能避免發炎產生降低對病人的副作用。然而近年來大量研究與臨床報告指出癌症細胞的凋亡路徑常常是突變缺陷或是最後對凋亡誘導的抗性，導致對化療效果不佳。自凋亡機制被發現至今，至少有12種以上的新穎程序性細胞死亡被報導，如何利用與調節這些新穎程序性細胞死亡可能成為重要癌症治療策略。
我們實驗室的主要興趣則是對於這些新穎程序性細胞死亡方式如何在癌症治療應用。1. 我們實驗室主要探索台灣常見的癌症包括肝癌、乳癌及非小細胞肺癌如何對臨床藥物產生抗性。2. 透過了解癌細胞產生抗藥性機制進行預防或發展使癌細胞對臨床藥物重新致敏化 (Chemosensitizer)的策略。3. 除了細胞凋亡 (Apoptosis)，我們也對新穎非凋亡性細胞死亡(Non-apoptotic programmed cell death)包括鐵凋亡 (Ferroptosis)、程序性壞死(Necroptosis)、細胞焦亡 (Pyroptosis)及類凋亡 (Paraptosis) 有興趣，希望能運用癌細胞本身特性以誘導細胞死亡以抑制癌細胞，並且能夠透過了解這些程序性的細胞死亡機制，未來就能運用在其他疾病的治療。
歡迎有興趣並積極努力的同學加入我們實驗室，一起發掘探索細胞死亡真相!!
{slider Non-apoptosis 非凋亡性的細胞死亡}
- 非凋亡性的細胞死亡特徵比較
- Methuosis 喝到中毒的一種細胞死亡?
文獻來源：https://ajp.amjpathol.org/article/S0002-9440(14)00183-7/fulltext 2014 American Journal Of Pathology 184(6)
Methuosis, derived from the Greek “methuo”, means to drink to intoxication. Large fluid-filled vacuoles derived from macropinosomes accumulated in the cytosol of methuotitc cells. Methuosis來自希臘語“methuo”，意思是喝到中毒。大量液泡堆積在Methuosis細胞的細胞質中。
Methuosis細胞死亡的細胞型態 (光學顯微鏡/穿透式電子顯微鏡/免疫螢光圖)
https://ajp.amjpathol.org/article/S0002-9440(14)00183-7/fulltext
{slider 實驗室與本校臨床教師共同合作的研究成果}
苯氧酚化合物diTFPP能透過調控肝細胞癌的外源性C2-神經醯胺代謝，並調節ROS的形成和自噬壓力而誘導肝癌細胞凋亡
[與本系合聘臨床醫學教師張文燦教授合作]
Antioxidants ( IF 5.014 ) DOI: 10.3390/antiox10030394 . 簡介：外源性合成短碳鏈神經醯胺可抑制癌細胞生長及誘導細胞死亡。然而細胞內醯胺代謝途徑會傾向將外源性C2-神經醯胺代謝成向存活性醯胺而導致C2-神經醯胺誘導的抗肝癌細胞作用減弱。 而苯氧酚化合物diTFPP可能協助調節細胞內鞘脂醯胺代謝，使C2-神經醯胺為促凋亡並增強ROS的產生和自噬壓力，並最終使肝細胞癌對於對C2-神經酰胺誘導凋亡致敏化。
除了癌症領域，細胞生與死的平衡失調是導致許多疾病產生主因，因此我們也探討疾病包括眼部退化預防探討薑黃素衍生物於藍光照射視網膜色素上皮層細胞的保護機制
糖尿病造成的視網膜損傷之小分子預防 [與本系合聘臨床醫學教師 許淑娟教授合作]
說明：利用氯奎寧(Chloroquine)破壞細胞自噬能產生肺癌細胞致敏效果[全文]
說明：喹啉小分子可以透過調控ERK 活性強度達到抑制肺癌移行能力或誘導凋亡 [全文]- 與醫化系陳義龍教授、藥學系曾志華教授合作成果。
粒線體對人體疾病影響的轉譯研究與預防策略 [與本校臨床醫學教師 林祐賢助理教授合作]
林祐賢醫師為腎臟內科主治醫師, 實驗室為針對粒線體於人體疾病所扮演角色的轉譯實驗室。粒線體是細胞能量工廠, 主要利用Krebs Cycle 產生ATP 提供細胞能量。粒線體有分內膜、外膜。藉由訊息調控, 訊息因子不單單只是存在於細胞質(cytoplasm), 更會進出粒線體, 藉由電子傳遞鍊(electron transport chain), 調控complex I-V, 影響了O2、H2O的製造。也會導致ROS的產生。現在的研究證據顯示、粒線體不單單只是扮演提供細胞能量, 更在細胞凋亡扮演重要角色。林醫師實驗室, 利用細胞、動物模式, 與加州大學爾灣分校王秉訓教授實驗室證明了粒線體AKT1訊息的調控, 在缺血再灌流(ischemia-reperfusion)急性腎損傷(acute kidney injury, AKI)會導致腎臟細胞的損傷與修復。本研究獲得Kidney International (impact factor=8.95, ranking 6/85, Q1) (Kidney Int. 2021 Apr;99(4):870-884).
Translational Research of Mitochondrial Metabolism in Human Diseases [Collaboration with Dr. Hugo Y.-H. Lin, MD, Ph.D. of KMU Hospital]
Dr. Lin is a physician-scientist of the Division of Nephrology, and his laboratory is focusing on translation research of the role of mitochondria in human diseases. Mitochondria are cellular energy factories, which mainly utilize the Krebs Cycle to generate ATP for providing cellular energy. Mitochondria have inner and outer membranes. Through the signal’s transduction, the molecular signal not only exists in the cytoplasm but also translocates into the mitochondria. Through the electron transport chain, it regulates complex I-V and affects the production of O2 and H2O. It can also lead to the generation of ROS. Current research evidence shows that mitochondria not only play a role in providing cellular energy but also play an important role in apoptosis. This study was awarded by Kidney International (impact factor=8.95, ranking 6/85, Q1)
實驗原理與Q&A
{slider 流式細胞儀的工作原理}
流式細胞儀的工作原理以功能 https://www.youtube.com/watch?v=EQXPJ7eeesQ
{slider 金屬離子螯合劑的用途}
金屬離子螯合劑 Chelating agents EDTA and EGTA https://www.aatbio.com/resources/faq-frequently-asked-questions/What-is-the-difference-between-EDTA-and-EGTA
EDTA: 主要針對 Ca/Mg/Fe
EGTA: 對 Ca離子有很高的親和性
用途：
1. 抑制酵素活性: 核酸酶需離子才有活性，加EDTA可抑制酵素活性以免核酸被降解 (如：抽RNA/DNA)
2. 測試離子在訊息傳遞或細胞反應扮演的角色 [例如 想知道 ER stress 過程是否有 Ca 參與，可加入 EGTA]
{slider 細胞密度對生長影響極大}
出處：https://jbcahs.sbvjournals.com/doi/JBCAHS/pdf/10.5005/jp-journals-10082-02230
這篇文章利用不同密度 培養Y79 視網膜纖維母瘤細胞的生長速度差異極大，Y79的doubling time在最低密度幾乎需要72小時, 但最高細胞密度只需要24小時，但48小時就到達極限
(有些細胞需要朋友一起生活才能長得好，但也不能長太滿。)
In summary, the cellular growth curves for two CNS tumors (C6 and Y79) were established by evaluating DT's population. This study concludes that the cell line’s growth curve pattern is dependent on the seeding density. The growth curves constructed from 4,000 cells/cm2 show a more expected growth curve pattern, including the lag phase, exponential phase, plateau phase, and death phase. Increasing the seeding density changes some properties of the cells. These changes are cell line-specific. It is thus suggested that researchers consider the effect of seeding density before running any experiments. This study also helps decide and choose the best cell number and does well with prior knowledge about this cell line.
{slider Western blot 疑難解決 Troubleshooting }
如果要偵測磷酸化蛋白，請避免用脫脂牛奶(改用BSA)，可以避免非專一性(多條band, 背景過黑) 的問題 Cons of non-fat milk Shouldn't be used to detect phosphorylated proteins as it contains the phosphoprotein casein which may react with the antibody resulting in non-specific binding and a high background*. https://blog.citeab.com/milk-bsa-blocking/
{slider Annexin V-positive 細胞一定就是凋亡細胞嗎?}
Flipping the dogma – phosphatidylserine in non-apoptotic cell death
過去認為Annexin V 染色可以確定細胞是否有進行細胞凋亡(apoptosis), 但更新的證據顯示非凋亡的程序性細胞壞死(Non-apoptotic necroptosis) 也可能被Annexin V 染色. 有興趣的同學請參考內文並約時間討論。
文獻來源：Shlomovitz et al., Cell Commun Signal17, 139 (2019). https://doi.org/10.1186/s12964-019-0437-0
{slider Lysotracker 染活細胞溶小體，但免疫染色可以使用嗎?}
Lysotracker 活細胞的溶小體染劑，使用簡便，但可以用在免疫螢光染色法?
一般而言，酸性胞器所需的pH梯度會被酒精或醛改變，因此LysoTracker 大多情況僅可用於活細胞，而不能用於固定組織。 但有些時候可以先用lysotracker標定溶小體，但一定要在固定液使用之前。
另外，也可以以用溶酶體標記物LAMP1 / LAMP2 /組織蛋白酶B來標記溶酶體。
Since the pH gradient needed to stain acidic compartments is altered by alcohol or aldehydes, LysoTracker can only be used in live cells and cannot be used in fixed tissues. Stains may be used for this, but only after they've been lysosome stained. Alternatively, several lysosomal markers LAMP1 / LAMP2/ cathepsin B, which can be stained with LC3, can be used to label lysosomes.
來源資料：https://www.researchgate.net/profile/Ritu-Varshney
{slider 線上教育課程 }
Coursera 癌症生物導論 (旁聽免費/用學校電子信箱註冊第一門課免費取得證書)
學術倫理教育課程 [教育部委託交通大學辦理線上學術倫理課程，全台灣包括高醫大研究生畢業必要條件]
如何避免學術倫理_ 避免抄襲發生 The Tadpole Paper Mill
科技部 誠信電子報 (避免違反學術倫理)
期刊審稿的標準與流程 [用主編的視角看論文投稿審查過程] https://www.editorialmanager.com/bcan/editor_instructions.asp?formID=1
線上資源
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參考文獻格式產生器 非常有用的工具，特別是大家常傷腦筋的網頁引用 (APA/MLA/Chicago/Vancouver)。 -
AI 英文編輯修改 可偵測論文內容拼音與文法，準備壁報摘要或論文都很實用，暫時免費請多加利用。
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Convert image to TIFF format 線上轉換解析度 投稿時，一般期刊都會要求高解析度的圖片(>300DPI)，最好在轉檔時就以高解析度輸出。若不行的話可試這個線上轉換程式!! 最好使用無壓縮 TIFF 的格式為佳 (雖然有些期刊也接受 JPG 破壞型的圖片壓縮格式) 。
研究成果
期刊論文: (可連結科技部查看最新論文) Publication since 2015
1. Wen-Tsan Chang†, Yung-Ding Bow†, Yen-Chun Chen, Chia-Yang Li, Jeff Yi-Fu Chen, Yi-Ching Chu, Yen-Ni Teng, Ruei-Nian Li, and Chien-Chih Chiu* (2021, Jan) The Phenoxyphenol Compound diTFPP mediates Exogenous C2-Ceramide Metabolism, inducing Cell Apoptosis accompanied by ROS Formation and Autophagy in Hepatocellular Carcinoma Cells. Antioxidants, 10(3), 394. 與附院臨床教師 張文燦醫師共同著作 (研究生鮑永定)
2. Kai-Chun Cheng, Yun-Tzu Hsu, Wangta Liu, Huey-Lan Huang, Liang-Yu Chen, Chen-Xi He, Shwu-Jiuan Sheu, Kuo-Jen Chen, Po-Yen Lee, Yi-Hsiung Lin*, Chien-Chih Chiu* (2021, Jan). The role of oxidative stress and autophagy in blue light-induced damage to the retinal pigment epithelium in zebrafish in vitro and in vivo. Int. J. Mol. Sci. 29;22(3):1338. doi: 10.3390/ijms22031338. 與附院臨床教師 鄭凱駿醫師共同著作 (研究生許云慈)
2. Chung Shou-Jie; Ganta Prasanna; Chang Yu-Lun; Kosuru Someswara; Chang Heng-Yi; Ding Shangwu; Chen, Hsuan-Ying*; Chien-Chih Chiu*; Wu Kuo-Hui* (2020, Dec). Titanium Complexes Bearing 2,6-Bis(o-hydroxyalkyl)pyridine Ligands in Vitro Cytotoxicity Against Triple-Negative Breast-Cancer Cells. Chinese Chemical Society. (Accepted).應化系陳喧應教授共同著作.
3. Yu-Wen Kao, Sheng-Kai Hsu, Jeff Yi-Fu Chen, I-Ling Lin, Kuo-Jen Chen, Po-Yen Lee, Hui-Suan Ng, Chien-Chih Chiu*, Kai-Chun Cheng* (2020, Dec). Curcumin metabolite tetrahydrocurcumin in the treatment of eye diseases. Int. J. Mol. Sci. 2021, 22(1), 212; https://doi.org/10.3390/ijms22010212. (第一作者為醫學系高鈺雯同學/醫技系徐聖凱同學)與臨床教師 鄭凱駿醫師共同著作
4. Wen-Tsan Chang, Yung-Ding Bow, Pei-Rong Fu, Chia-Yang Li, Chang-Yi Wu, Yi-Hua Chang, Yen-Ni Teng, Ruei-Nian Li, Mei-Chin Lu, Yi-Chang Liu*, Chien-Chih Chiu* (2020, Nov). A marine terpenoid, heteronemin, induces both the apoptosis and ferroptosis of hepatocellular carcinoma cells and involves the ROS and MAPK pathways. Oxid Med Cell Longev. (Accepted). 與附院臨床教師 張文燦醫師共同著作
5. Feng-Jie Lai, Tsai-Wen Huang, Yu-Lun Chang, Heng-Yi Chang Chang, Wei-Yi Lu, Shangwu Ding, Hsuan Ying Chen*, Chien-Chih Chiu*, Kuo-Hui Wu* (2020, Jul). Titanium complexes bearing 2,6-Bis(o-hydroxyalkyl)pyridine ligands in the ring-opening polymerization of L-Lactide and ε-caprolactone. Polymer. 204, 122860.應化系陳喧應教授共著.
6. Kai-Hsu Sheng, Wen-Tsan Chang, I-Ling Lin, Yih-Fung Chen, Nitin Balkrushna Padalwar, Kai-Chun Cheng, Yen-Ni Teng, Chi-huei Wang *, Chien-Chih Chiu* (2020, Jul). The role of necroptosis in ROS-mediated cancer therapies and its promising applications. Cancers, 12(8), 2185. (第一作者為醫技系 徐聖凱同學)
7. Peng SY, Hsiao CC, Lan TH, Yen C, Farooqi AA, Cheng CM, Tang JY, Yu TJ, Yeh YC, Chuang YT, Chien-Chih Chiu* and Chang HW* (2020, Jun). Pomegranate extract inhibits migration and invasion of oral cancer cells by downregulating matrix metalloproteinase-2/9 and epithelial-mesenchymal transition. Environ. Toxicol, 35(6):673-678.
8. Hurng-Wern Huang, Yung-Ding Bow, Chia-Yih Wang, Yen-Chun Chen, Pei-Rong Fu, Kuo‑Feng Chang, Tso-Wen Wang, Chih-Hua Tseng, Yeh-long Chen*,and Chien-Chih Chiu* (2020, May)* DFIQ, a novel quinoline derivative shows anticancer potential by inducing apoptosis and autophagy in NSCLC cell and in vivo zebrafish xenograft models. Cancers 12(5), 1348. [全文] 與應用化學系 陳義龍教授共同著作
9. Wangta Liu, Chang-Yi Wu, Mei-Jei Lu, Yung-Jen Chuang, Eing Mei Tsai, Steve Leu, I-Ling Lin, Chih-Jan Ko*, Chien-Chih Chiu*, and Wen-Tsan Chang* (2020, Jan). Phenoxyphenol Compound 4-HPPP Selectively Induces Antiproliferation Effects and Apoptosis in Human Lung Cancer Cells through Aneupolyploidization and ATR DNA Repair Signaling. Oxid Med Cell Longev, 7;2020:5167292.
11. Wangta Liu, Yu-Lun Lo, Chin Hsu, Yi-Ting Wu, Zi-Xian Liao, Wen-Jeng Wu, Yi-Jou Chen, Chieh Kao, Chien-Chih Chiu*, and Li-Fang Wang*Contributed to this work equally. (2019, Sep). CS-PEI/Beclin-siRNA Downregulate Multidrug Resistance Proteins and Increase Paclitaxel Therapeutic Efficacy against NSCLC. Molecular Therapy — Nucleic Acids, 6; 17: 477–490.
12. Sheng-Kai Hsu†, Chien-Chih Chiu†, Hans-Uwe Dahms, Chon-Kit Chou, Chih-Mei Cheng, Wen-Tsan Chang, Kai-Chun Cheng, Hui-Min David Wang and I-Ling Lin 2*. † Equal contribution. (2019, May). Unfolded Protein Response (UPR) in Survival, Dormancy, Immunosuppression, Metastasis, and Treatments of Cancer Cells. Int. J. Mol. Sci, 20(10), 2518. †Contributed equally (第一作者為醫技系 徐聖凱同學/)
13. Han-Lin Chou, Yi-Hsiung Lin, Wangta Liu, Chang-Yi Wu, Ruei-Nian Li, Hurng-Wern Huang, Chi-Hsien Chou, Shean-Jaw Chiou, Chien-Chih Chiu*. Combination therapy of chloroquine and C2-ceramide enhanced cytotoxicity in Lung cancer H460 and H1299 cells. Cancers. 2019. 11(3):370. (第一作者為 中山大學生物醫學研究所 周翰林)
14. Chon-Kit Chou, Hurng-Wern Huang, Chun-Feng Yang, Hans-Uwe Dahms, Shih-Shin Liang, Tsu-Nai Wang, Po-Lin Kuo, Edward Hsi, Eing-Mei Tsai*, Chien-Chih Chiu*. Reduced camptothecin sensitivity of estrogen receptor-positive human breast cancer cells following exposure to di(2-ethylhexyl)phthalate (DEHP) is associated with DNA methylation changes. Environ. Toxicol, 2019 34(4):401-414. 與附院臨床教師 蔡英美醫師共同著作
15. CM Chien, JC Yang, PH Wu, CY Wu, GY Chen, YC Wu, CK Chou, CH Tseng, YL Chen*, Li-Fang Wang*, Chien-Chih Chiu*. Phytochemical Naphtho[1,2-b] furan-4,5-dione Induced Topoisomerase II-mediated DNA Damage Response in Human Non-small-Cell Lung Cancer. Phytomedicine. 2019, 54: 109-119
16. Feng-Chi Hsieh, Chun-Tzu Hung, Kai-Chun Cheng, Chang-Yi Wu, Yen-Chun Chen, and Yu-Jen Wu, Wangta Liu*, Chien-Chih Chiu*. Protective effects of Lycium barbarum extracts on UVB-induced damage in human retinal pigment epithelial cells accompanied by attenuating ROS and DNA damage. Oxid Med Cell Longev. 2018. Article ID 4814928
17. YC. Chang, Y Fong, EM Tsai, YG Chang, HL Chou, CY Wu, YN Teng, TC Liu, SS Yuan, and Chien-Chih Chiu*. Exogenous C8-ceramide induces apoptosis by over-production of ROS and the switch of superoxide dismutases SOD1 to SOD2 in human lung cancer cells. Int. J. Mol. Sci. 2018. 19(10), 3010.
18. Chon-Kit Chou, Wangta Liu, Yu-Jie Hong, Hans-Uwe Dahms, Chen-Hao Chiu, Wen-Tsan Chang, Ching-Ming Chien, Chia-Hung Yen, Yuan-Bin Cheng*, Chien-Chih Chiu*. Ethyl acetate extract of Scindapsus cf. hederaceus exerts inhibitory bioactivity on human non-small cell lung cancer cells through modulating ER stress. Int. J. Mol. Sci. 2018, 19.7: 1832.
19. YH CHIU, SH HSU, HW HSU, KC HUANG, W LIU, CY WU, WP HUANG, JEFF YF CHEN, BH CHEN, and Chien-Chih Chiu*. Human lung cancer cells can be sensitized to camptothecin by modulating autophagy. Int J Oncol. 2018, 53.5: 1967-1979.
20. Chon-Kit Chou, Ya-Ting Yang, Ho-Chun Yang, Shih-Shin Liang, Tsu-Nai Wang, Po-Lin Kuo, Hui-Min David Wang, Eing-Mei Tsai*, Chien-Chih Chiu*. The impact of Bis(2-ethylhexyl) phthalate (DEHP) on cancer progression. Archivum Immunologiae et Therapiae Experimentalis. 2017 66.3: 183-197.
21. WT Chang, W Liu, YH Chiu, BH Chen, SC Chuang, YC Chen, YT Hsu, MJ Lu, SJ Chiou, CK Chou, and Chien-Chih Chiu*. A 4-Phenoxyphenol Derivative Exerts Inhibitory Effects on Human Hepatocellular Carcinoma Cells through Regulating Autophagy and Apoptosis Accompanied by Downregulating α-Tubulin Expression. Molecules, 2017, 22,854.
22. Chang WT, Fong Y, Chuang SC, Chou CK, Chou HL, Yang CF, Tseng CH, Chen YL,Chien-Chih Chiu*. 9-bis[2-(pyrrolidin-1-yl) ethoxy] -6-{4-[2-(pyrrolidin-1-yl) ethoxy]phenyl}-11H-indeno [1,2-c]quinolin-11-one (BPIQ), a quinoline derivative inhibits human hepatocellular carcinoma cells by inducing ER stress and apoptosis. Anticancer Agents Med Chem.2017;17(5):692-700.
23. Wen-Tsan Chang, Wangta Liu, Yi-Han Chiu, Bing-Hung Chen, Shih-Chang Chuang, Yen-Chun Chen, Yun-Tzh Hsu, Mei-Jei Lu, Shean-Jaw Chiou, Chon-Kit Chou, and Chien-Chih Chiu*. A 4-Phenoxyphenol Derivative Exerts Inhibitory Effects on Human Hepatocellular Carcinoma Cells through Regulating Autophagy and Apoptosis Accompanied by Downregulating α-Tubulin Expression. Molecules. 2017 May 21;22(5)
24. Chon-Kit Chou, Chin-Ju Tang, Han-Lin Chou, Chun-Yen Liu, Ming-Chong Ng, Yu-Ting Chang, Shyng-Shiou F. Yuan, Eing-Mei Tsai, Chien-Chih Chiu*. The Potential Role of Krüppel-Like Zinc Finger Protein Glis3 in Genetic Diseases and Cancers. Arch Immunol Ther Exp (Warsz). 2017 May 18
25. Fong Y, Wu CY, Chang KF, Chen BH, Chou WJ, Tseng CH, Chen YC, Wang HD, Chen YL, Chien-Chih Chiu*. Dual roles of the extracellular signal-regulated kinase (ERK) in quinoline compound BPIQ-induced apoptosis and anti-migration of human non-small cell lung cancer cells. Cancer Cell Int. 2017 Mar 7;17:37.
26. Chon-Kit Chou, Yu-Ting Chang, Michal Korinek, Yei-Tsung Chen, Ya-Ting Yang, Steve Leu, I-Ling Lin, Chin-Ju Tang, Chien-Chih Chiu*. The regulations of deubiquitinase USP15 and its pathophysiological mechanisms in diseases. Int J Mol Sci. 2017 Feb 24;18(3)
27. Chou HL, Fong Y, Lin HH, Tsai EM, Chen YF, Chang WT, Wu CY, Wang HM, Huang HW, and Chien-Chih Chiu * An acetamide derivative as a camptothecin sensitizer for human non-small cell lung cancer cells through increased oxidative stress and JNK activation". Oxid Med Cell Longev. 2016. Article ID 9128102.
28. Fong Y, Tang CC, Hu HT, Fang HY, Wu CY, Yuan SS, Wang HM, Chen YC, Teng YN, and Chien-Chih Chiu *. The inhibitory effect of trans-Ferulic acid on proliferation and migration of human lung cancer cells through modulating endogenous ROS and beta-catenin activity. Chinese Medicine, 2016 11:45. eCollection 2016.
29. Liu YS, Cheng RY, Lo YL, Hsu C, Chen SH, Chien-Chih Chiu*, Wang LF*. (*Corresponding authors) (2016, Jan). Distinct CPT-induced deaths in lung cancer cells caused by clathrin-mediated internalization of CP micelles. Nanoscale, 8(6):3510-22.
30. Chien-Chih Chiu*, Kuo-Feng Chang, Bing-Hung Chen, Han-Lin Chou, Chih-Hua Tseng, Yao Fong, Tzu-Fun Fu, Hsueh-Wei Chang, Chang-Yi Wu, Eing-Mei Tsai, Shinne-Ren Lin and Yeh-Long Chen* (*Corresponding authors) (2015, Dec). BPIQ, a novel synthetic quinoline derivative, inhibits growth and induces mitochondrial apoptosis of lung cancer cells in vitro and in zebrafish xenograft model. BMC Cancer, 15:962.
31. Chih-Hua Tseng, You-Ren Chen, Cherng-Chyi Tzeng, Wangta Liu, Chon-Kit Chou, Chien-Chih Chiu*, Yeh-Long Chen* (*Corresponding authors) (2015, Nov). Discovery of Indeno[1,2-b]quinoxaline Derivatives as Potential Anticancer Agents. European Journal of Medicinal Chemistry.
32. Kai-Chun Cheng, Chun-Tzu Hung, Kuo-Jen Chen, Wen-Chuan Wu, Jau-Ling Suen, Cheng-Hsien Chang, Chi-Yu Lu, Chih-Hua Tseng, Yeh-Long Chen, Chien-Chih Chiu* (2015, Aug). Quinoline-Based Compound BPIQ Exerts Anti-Proliferative Effects on Human Retinoblastoma Cells via Modulating Intracellular Reactive Oxygen Species. Arch Immunol Ther Exp (Warsz). 2015 Apr;64(2):139-47
33. Chon-Kit Chou, Chang-Yi Wu, Jeff Yi-Fu Chen, Ming‐Chong Ng, Hui-Min David Wang, Jen-Hao Chen, Shyng-Shiou F Yuan, Eing-Mei Tsai, Jan-Gowth Chang*, Chien-Chih Chiu* (2015, Jul). BubR1 Acts as a Promoter in Cellular Motility of Human Oral Squamous Cancer Cells through Regulating MMP-2 and MMP-9. International Journal of Molecular Sciences, 16(7):15104-17.
Conferences
· Han-Lin Chou, Hurng-Wern Huang, Chien-Chih Chiu*. Chloroquine synergistically promotes C2-ceramide-induced apoptosis of non-small cell lung cancer cells via inhibiting sirt1 expression. 2013 Taiwan Zebrafish Symposium Dec. 7. Institute of Biomedical Sciences Academia Sinica. #P22. (The best poster award). 台灣斑馬魚研討會
· Mei-Jei Lu and Chien-Chih Chiu*. The effect of Akt-targeting compounds on proliferation and apoptosis of lung cancer cells in zebrafish xenografts.2013 Taiwan Zebrafish Symposium Dec. 7. Institute of Biomedical Sciences Academia Sinica.#P23. 台灣斑馬魚研討會
· Chien Chih, Chiu, and Bing-Hung Chen*. Anti-allergic activity of grape seed extract (GSE) on RBL-2H3 mast cells. The 19th Symposium of Young Asian Biochemical Engineer’s Community (YABEC). (ORAL#1). 2013. Urumqi, China.
· Chon Kit, Chou, Chien Chih, Chiu*. The 19th Symposium of Young Asian Biochemical Engineer’s Community (YABEC). (POSTER#PD1). YABEC 2013. Urumqi, China. (YABEC *best poster award)
· Han Lin, Chou, Chien Chih, Chiu*. The 19th Symposium of Young Asian Biochemical Engineer’s Community (YABEC). (POSTER#PD2). 2013. Urumqi, China.
· Yin-Chieh Lin, Jeff Yi-Fu Chen, Chien-Chih Chiu*. The inhibitory effects of sirtinol, a sirtuin inhibitor on invasion of non-small cell lung cancer cells via Src signaling. The 18th Symposium of Young Asian Biochemical Engineer’s Community (YABEC) 2012. (ORAL: O-CD-7).
· Pin-Hsuan Wu, Jeff Yi-Fu Chen, Yeh-Long Chen, Chien-Chih Chiu*. Naphtho[1,2-b] furan-4,5-dione, a naphthoquinone derivative induces anti-cell growth and anti-migration in non-small cell lung cancer by inhibiting topoisomerase II. Bio-IT WORLD ASIA in Marina Bay Sands Hotel, Singapore, in June 6-8, 2012. (*corresponding). (POSTER #6) Session of Drug Discovery and Bioinformatics.
· Yuli C. Chang, Shih-Ya Hung, Chien-Chih Chiu, Wen-Lin Chan, Wen-Hsin Chang, Shou-Mei Wu, Ya-Sian Chang, Chung-Yee Yuo, Ta-Chih Liu, Wen-Kuang Yang, Jan-Gowth Chang*. Participation of an XIST-related small RNA in the formation of the K-RAS G-quadruplex. Non-Coding RNAs (Z1) Keystone Symposia conference. Snowbird, Utah, USA, in March 31-April 5. 2012
· Yin-Chieh Lin, Han-Lin Chou, Chien-Chih Chiu＊. The inhibitory effects of sirtuin1 inhibitor, sirtinol on migration and anchorage-independent growth of lung cancer cells. (POSTER #85) SCMB2012. 第20 屆細胞及分子生物新知研討會 (*corresponding)
· Han-Lin Chou, Feng-Wei Chen, Xin-Ping Tang, Hurng-Wern Huang＊, Chiu, Chien-Chih＊. The synergistic effect of C2-ceramide in Doxorubicin was induced senescence of non-small-cell lung cancer (NSCLC). (POSTER #57) SCMB2012. 第20 屆細胞及分子生物新知研討會 (*corresponding)
· Chon Kit Chou, Han-Lin Chou, Feng-Wei Chen, Chien-Chih Chiu*.The Novel Function of BubR1 in Migration Ability of Non-Small Cell Lung Cancer Cells. (POSTER #121) SCMB2012. 第20 屆細胞及分子生物新知研討會(*corresponding)
· Huei-Ting Hu, Hsueh-Wei Chang*, Lian-Je Hwang, Hsin-Yu Fang, Chien-Chih Chiu*. Effect of ferulic acid on anti-oxidation and cell proliferation of human lung cancer cells. (POSTER #95) SCMB 2012. 第20 屆細胞及分子生物新知研討會. (*corresponding)
· Pin-Hsuan Wu, Chien-Chih Chiu*, Yeh-Long Chen, Hsueh-Wei Chang. A novel naphthoquinone derivative-induced apoptosis and growth inhibition of human non-small cell lung cancer (NSCLC). (POSTER #27) SCMB2012. 第20 屆細胞及分子生物新知研討會. (*corresponding)
· Chiu-Chien Chiu*, Chon-Kit Chou, Han-Lin Chou. Goniothalamin inhibits the growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability. (Oral and POSTER) (POSTER # F14). The 17th Symposium of Young Asian Biochemical Engineer’s Community (YABEC) in Incheon, Korea on Oct 7-9,2011. (*corresponding)
· Kuo-Feng Chang, Hsueh-Wei Chang, Tso-Wen Wang, Chih-Hua Tseng, Yeh-Long Chen, Chien-Chih Chiu*. The mechanistic study of quinoline-derivative-induced apoptosis and growth inhibition of lung cancer. (POSTER #160) SCMB 2011. 第19屆細胞及分子生物新知研討會. (*corresponding)
· Kuo-Feng Chang, Hsueh-Wei Chang, Wan-Ju Chou, Chih-Hua Tseng, Yeh-Long Chen, Chien-Chih Chiu*. The quinoline-derivative DFIQ induced apoptosis and growth inhibition of lung cancer. SCMB 2011. 第19屆細胞及分子生物新知研討會. (*corresponding)
· Han-Lin Chou, Hsueh-Wei Chang, Pi-Chuan Hsieh, Xin-Ping Tang, Feng-Wei Chen, Hurng-Wern Huang*, Chiu, Chien-Chih*. Role of exogenous ceramides in modulating the expression of human type III deacetylase SIRT-1 and SIRT-2 of human non-small cell lung cancer (NSCLC) cells. (POSTER #86) SCMB 2011.第19屆細胞及分子生物新知研討會. (*corresponding)
· Yin-Chieh Lin, Jeff Yi-Fu Chen, Hsueh-Wei Chang*, Chien-Chih Chiu*. The modulation of cell proliferation induced by exogenous ceramide in human breast cancer cells. (POSTER #114) SCMB 2011. 第19屆細胞及分子生物新知研討會. (*corresponding)
· Huei-Ting Hu, Hsueh-Wei Chang, Lian-Je Hwang, Hsin-Yu Fang, Chien-Chih Chiu*. Effect of feruloyl xylooligosaccharides on antioxidant activity and cell migration in human lung cancer H1299 cells. (POSTER #133) SCMB 2011. 第19屆細胞及分子生物新知研討會. (*corresponding)
· Pin-Hsuan Wu, Chien-Chih Chiu*, Yeh-Long Chen, and Hsueh-Wei Chang*. NFD inhibits cell proliferation and migration of human non-small cell lung cancer (NSCLC) cell through DNA damage and reduced migration ability. (POSTER #50) SCMB 2011. 第19 屆細胞及分子生物新知研討會. (*corresponding)
· Chon-kit Chou, Han-Lin Chou, Pin-Hsuan Wu, Hsueh-Wei Chang* and Chien-Chih Chiu*. The effect of spindle assembly checkpoint kinase BubR1 on cellular migration in oral squamous cell carcinoma. (POSTER #171) SCMB 2011. 第19 屆細胞及分子生物新知研討會. (*corresponding)
· Kuang-Jing Huang, Chien-Chih Chiu*, Fang-Rong Chang, Yang-Chang Wu, Hsueh-Wei Chang*. AN10 is a potential natural product-derived drug against oral cancer. SCMB 2011. 第19 屆細胞及分子生物新知研討會.(*corresponding)
· Kuang-Jing Huang, Chien-Chih Chiu*, Fang-Rong Chang, Yang-Chang Wu, Hsueh-Wei Chang*. AN10 inhibits the growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability. (POSTER #256) SCMB 2011. 第19屆細胞及分子生物新知研討會. (*corresponding)
· Kuang-Jing Huang, Chien-Chih Chiu*, Fang-Rong Chang, Yang-Chang Wu, Hsueh-Wei Chang*. SCMB 2011. AN37 is a potential natural product-derived drug against oral cancer.第19屆細胞及分子生物新知研討會.
· Jeff Yi-Fu Chen, Chien-Chih Chiu, Yu-Jen Wu, Hau-Ren Chen. Betel Quid Extract Promotes Oral Cancer Cell Migration by Activating a Muscarinic M4 Receptor-Mediated Signaling Cascade Involving ERK1/2 and SFKs. 4th World Congress of International Federation of Head and Neck Oncologic Societies in Seoul, Korea on June 15-19, 2010.
· Guang-Jing Huang, Ming-Tz Tsai, Chon-Kit Chou, Chien-Chih Chiu, Yang-Chang Wu, Hsueh-Wei Chang. Growth inhibition of lung cancer cell using herb-derived compound AN10 based on cell migration analysisSCMB 2010. 第18屆分子生物學會冬令營
· Yung-Lan Su, Yi-Han Lo, Wan-Yu Chen, Tsung-Lun Kuo, Jan-Gowth Chang*, Chien-Chih Chiu*.Characterization of ectopic expression of nuclear export factor (NXF) in human hepatoma cells Huh-7 (POSTER # P331) The Twenty-fourth Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan. (Mar. 21-22, 2009). 第24屆生物醫學聯合學術年會 (*corresponding)
· Chien-Chih Chiu* (Oral Presentation # O46 ). Effects of ectopically expressed nuclear export factor (NXF) on cell migration and adhesion in human hepatoma cells. The Twenty-fourth Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan, Mar. 21-22, 2009. 第24屆生物醫學聯合學術年會 (*oral and corresponding)
· Pi-Chuan Hsieh, Chien-Chih, Chiu, Jan-Gowth Chang, Sheng-Fung Lin.BubR1 Gene Is Involved in Ceramide Induced Mitotic Arrest in Malignant Cancer Cells. (May 2007). 第12屆台灣癌症聯合學術年會
· Ying-Chieh Chu, Kai-Li Su, Min-Tsui Wu, Chien-Chih Chiu*. The effects of caffeic acid in anticancer drug-induced human non-small cell lung cancer (NSCLC) cells. (Mar 2007). 第22屆生物醫學年會 (*corresponding)
實驗室榮譽榜
校內暑期專題：
109學年度
學生暑期參與專題研究補助 <研究成果報告績優> 3/31 (全校只取 31名，本實驗室有三位得獎)
https://devel.kmu.edu.tw/images/Div_AR/1015005/summer_research/109-top.pdf (研發處網頁)
1. S109119-3 陳姸凝 生物醫學暨環境生物學系 (與張學偉老師共同指導)
- 新穎 nitrated [6,6,6]tricycles衍生物SK1的抗口腔癌生長抑制與誘導細胞凋亡的機制探討
2. S109119-4 何嘉紜 探討長期塑化劑暴露造成三陰性乳癌細胞粒線體膜電位增加與細胞行為改變
3. S109119-2 周思燕 探討喹啉類小分子透過調節鐵凋亡與細胞凋亡抑制人類非小細胞肺癌與評估其免疫治療應用
4. 2020 #43 高鈺雯 (醫學系三) 校內大學生研究計畫成果壁報論文競賽 優勝 https://reurl.cc/VXQMRR !! (研發處網頁)
|
計畫編號 |
姓名 |
科技部大專生專題計畫名稱 |
|
101-2815-C-037-030-B |
陳豐煒 |
塑化劑DEHP對肺癌細胞及血管新生的影響機制探討 |
|
102-2815-C-037-029-B |
黃鼎文 |
探討短碳鏈神經醯胺調控人類肺癌細胞內去乙醯化酵素SIRT1表現機制 |
|
107-2813-C-037-047-B |
曹立儀 |
評估紅石榴與紅石榴素對口腔癌細胞的生長與移動的調節作用 |
|
107-2813-C-037-060-B |
謝慶宏 |
探討USP15促進非小細胞肺癌獲得化療抗藥性之機制 (107年研究創作獎) |
|
108-2813-C-037-044-B |
陳柏安 |
探索鬍鬚地衣甲醇萃取物利用氧化壓力毒殺口腔癌細胞的機制 |
|
108-2813-C-037-077-B |
張乙樺 |
海綿天然物Heteronemin 對於誘導頭頸癌細胞凋亡之機制與路徑間的探討 |
招募實驗室生力軍！歡迎願意主動學習，積極有耐心的同學加入我們！校內外皆可不限系級，有熱誠肯吃苦都歡迎! 外校同學歡迎暑期實習並會適度提供津貼
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申請辦法與時程：詳見研發處網頁
https://devel.kmu.edu.tw/index.php/zh-TW/學術研究組/大學生研究計畫/57-大學生暑期研究-校內補助
招募生力軍 Internship
招募實驗室生力軍！歡迎願意主動學習，積極有耐心的同學加入我們！校內外皆可不限系級，有熱誠肯吃苦都歡迎! 外校同學歡迎暑期實習並會適度提供津貼
有興趣的校內同學執行暑期計畫(高醫大限升大二與升大三的同學)
請在5/28 (研發處截止) 與我聯繫 cchiu@kmu.edu.tw #2368，盡早討論可優先挑選自己有興趣的題目。
★執行暑期研究並繳交成果報告補助8000元
★參加8月底研究計畫撰寫講座+1000元
★申請111年度科技部研究計畫+1000元
★參加9~10月研究計畫成果壁報論文競賽獎金優渥$$$$$
申請辦法與時程：詳見研發處網頁
https://devel.kmu.edu.tw/index.php/zh-TW/學術研究組/大學生研究計畫/57-大學生暑期研究-校內補助
為何參與校內暑期研究計畫?? [詳見本校研發處網站]
您想一探實驗室的神秘面紗嗎?
您想學習撰寫與執行研究計畫的方法嗎? 想從做中學，累積實作能力嗎?
您想成功取得科技部大專學生研究計畫嗎?
執行校內暑期研究計畫是您進入研究領域的最佳途徑~
請想體驗作中學研究及目標推甄研究所的學生，千萬不要錯過囉!!
科技部大專生研究計畫 ( 每年的一月底至二月) - [詳見本校研發處網站]
申請計畫獲通過之學生，科技部補助4.8萬元（8個月），
科技部相關連結網址
https://www.most.gov.tw/
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