聯絡方式

• 辦公室: 第一教學大樓N1035室
• 實驗室: 第一教學大樓N1116室(生化分析實驗室)
• 電話: (07) 3121101 ext. 2153
• 傳真: (07) 3125339
• 電子郵件: liang0615@kmu.edu.tw

學經歷

• 國立台灣大學化學系理學士 (1995/9-1999/6)

• 國立成功大學化學研究所化學博士 (2003/9-2009/6)

• 國立成功大學化學研究所博士後研究員 (2009/8-2010/7)

• 高雄醫學大學生物科技學系助理教授 (2010/8-2015/7)

• 高雄醫學大學研究資源整合發展中心組長(2011/8-2015/7)

• 高雄醫學大學生物科技學系副教授 (2015/8-2019/7  )

• 高雄醫學大學生物科技學系教授 (2019/8-  )

主負責授課科目

• 生物物理化學、生物分析方法、奈米生物科技、質譜學
• 儀器分析特論

 研究興趣

• 質譜暨管柱分離層析
• 高分子與奈米複合材料

近期研究成果

期刊論文

• De-Cheng Tsai, Meng-Chieh Liu, Yi-Reng Lin, Mei-Fang Huang, Shih-Shin Liang* (2016, Jan). A novel reductive amination method with isotopic formaldehydes for the preparation of internal standard and standards for determining organosulfur compounds in garlic. Food Chemistry, 197, 692–698. (SCI, 8/123, FOOD SCIENCE & TECHNOLOGY). MOST 102-2113-M-037-013-MY2. 本人為通訊作者.

• Kung-Kai Kuo,* Chao-Jen Kuo, Chiang-Yen Chiu, Shih-Shin Liang, Chun-Hao Huang, Shu-Wen Chi, Kun-Bow Tsai, Chiao-Yun Chen, Edward Hsi, Kuang-Hung Cheng and Shyh-Horng Chiou* (2016, Jan). Quantitative Proteomic Analysis of Differentially Expressed Protein Profiles Involved in Pancreatic Ductal Adenocarcinoma. Pancreas, 45, 71-83. (SCI, 31/76, GASTROENTEROLOGY & HEPATOLOGY). MOST 101-2314-B-037-028-MY3.

• Shih-Shin Liang , Tsu-Nai ∗ Wang, Chien-Chih Chiu, Po-Lin Kuo, Mei-Fang Huang,Meng-Chieh Liu, Eing-Mei Tsai* (2016, Jan). Reductive aminationassisted quantitation of tamoxifen and itsmetabolites by liquid phase chromatography tandem mass spectrometry. Journal of Chromatography A, 1434, 64-69. (SCI, 6/78, analytical chemistry). MOST 104-2113-M-037-005-MY2. 本人為第一作者、通訊作者.

• Tsung-Hua Hsieh, Chia-Yi Hsu, Cheng-Fang Tsai, Chien-Chih Chiu, Shih-Shin Liang, Tsu-Nai Wang, Po-Lin Kuo, Cheng-Yu Long, Eing-Mei Tsai* (2016, Jan).
  A novel cell-penetrating peptide suppresses breast tumorigenesis by inhibiting β- catenin/LEF-1 signaling. Scientific Reports, 6:19156. (SCI, 5/57, MULTIDISCIPLINARY SCIENCES). MOST 104-2314-B-037-091.

• Yi-Chun Yeh, Mei-Feng Huang, Shih-Shin Liang, Shang-Jyh Hwang, Jer-Chia Tsai, Tai-Ling Liu, Ping-Hsun Wu, Yi-Hsin Yang, Kuang-Che Kuo, Mei-Chuan Kuo*, Cheng-Sheng Chen* (2016, Jan). Indoxyl sulfate, not p-cresyl sulfate, is associated with cognitive impairment in early-stage chronic kidney disease. NeuroToxicology, 53, 148-152. (SCI, 20/88, TOXICOLOGY). MOST 102-2314-B-037-007.

• Hugo You-Hsien Lin, Chi-Chih Hung, Yu-Han Chang, Ming-Yen Lin, Ming-Yu Yang, Shih-Shin Liang, Wangta Liu, Hung-Chun Chen, Shang-Jyh Hwang (2015, Oct). Nonapnea Sleep Disorders in Patients Younger than 65 Years Are Significantly Associated with CKD: A Nationwide Population-Based Study. PLOS ONE, journal.pone.0140401. (SCI, 9/57, MULTIDISCIPLINARY SCIENCES).

• Meng-Chieh Liu, Yi-Reng Lin, Mei-Fang Huang, De-Cheng Tsaid, Shih-Shin Liang* (2015, Jun). Mass spectrometry signal enhancement by reductive amination. International Journal of Mass Spectrometry, 387, 16-23. (SCI, 20/44, SPECTROSCOPY). 本人為通訊作者.

• Wangta Liu, Yow-Ling Shiue, Yi-Reng Lin, Hugo You-Hsien Lin and Shih-Shin Liang* (2015, May). A Derivative Method with Free Radical Oxidation to Predict Resveratrol Metabolites by Tandem Mass Spectrometry. Current Analytical Chemistry, 11(4), 300-306. (SCI, 55/74, CHEMISTRY, ANALYTICAL). MOST 102-2113-M-037-013-MY2. 本人為通訊作者.

• Chien-Feng Li, Wen-Jeng Wu, Wen-Ren Wu, Yu-Jing Liao, Lih-Ren Chen, Chun-Nung Huang, Ching-Chia Li, Wei-Ming Li, Hsuan-Ying Huang, Yi-Ling Chen, Shih-Shin Liang, Nan-Haw Chow, Yow-Ling Shiue* (2015, Apr). The cAMP responsive element binding protein 1 transactivates epithelial membrane protein 2, a potential tumor suppressor in the urinary bladder urothelial carcinoma. Oncotarget, 6(11), 9220-9239. (SCI, 21/211, ONCOLOGY). MOST 103-2314-B-110-001.

• Shyh-Horng Chiou*, Chun-Hao Huang, Shih-Shin Liang (2015, Mar). From Chemistry to Translational Medicine: The Application of Proteomics to Cancer Biomarker Discovery and Diagnosis. JOURNAL OF THE CHINESE CHEMICAL SOCIETY, 62(3), 217-226. (SCI, 127/157, CHEMISTRY, MULTIDISCIPLINARY). MOST 102-2320-B-037-025.

• Chun-Hao Huang, Fang-Yu Hsu, Yuan-Heng Wu, Linda Zhong, Mu-Yun Tseng, Chao-Jen Kuo, Ao-Lin Hsu*, Shih-Shin Liang*, Shyh-Horng Chiou* (2015, Feb). Analysis of lifespan-promoting effect of garlic extract by an integrated metabolo-proteomics approach. Journal of Nutritional Biochemistry, 26(8), 808-817. (SCI, 14/77, NUTRITION & DIETETICS). MOST 99-2745-B-037-005. 本人為通訊作者.

• Chieh-Yu Liao, Chia-Lin Lee, Hui-Chun Wang, Shih-Shin Liang, Po-Hsiung Kung, Yang-Chang Wu, Fang-Rong Chang, Chin-Chung Wu* (2015, Jan). CLL2-1, a chemical derivative of orchid 1,4-phenanthrenequinones, inhibits human platelet aggregation through thiol modification of calcium-diacylglycerol guanine nucleotide exchange factor-I (CalDAG-GEFI). FREE RADICAL BIOLOGY AND MEDICINE, 78, 101-110. (SCI, 16/128, ENDOCRINOLOGY & METABOLISM). MOST 98-2320-B-037-013-MY3.

• Shih-Shin Liang, Tsu-Nai Wang and Eing-Mei Tsai (2014, Nov). Analysis of Protein–Protein Interactions in MCF-7 and MDA-MB-231 Cell Lines Using Phthalic Acid Chemical Probes. International Journal of Molecular Sciences, 15, 20770-20788. (SCI, 52/148). MOST 100-2113-M-037-012-MY2. 本人為第一作者、通訊作者.

• Yi-Reng Lin, Chao-Jen Kuo, Hugo You-Hsien Lin, Chin-Jen Wu and Shih-Shin Liang (2014, Aug). A Proteomics Analysis to Evaluate Cytotoxicity in NRK-52E Cells Caused by Unmodified Nano-Fe3O4. The Scientific World Journal, http://dx.doi.org/10.1155/2014/754721. (SCI, 16/55, MULTIDISCIPLINARY SCIENCES). 本人為通訊作者.

• Chien-Feng Li, Hsuan-Ying Huang, Wen-Ren Wu, Shih-Shin Liang,Yi-Ling Chen, Lih-Ren Chen, Yu-Ting Peng, Hui-Chieh Lee and Yow-Ling Shiue (2014, Jun). Clinical Aggressiveness of Myxofibrosarcomas Associates with Down- Regulation of p12CDK2AP1: Prognostic Implication of a Putative Tumor Suppressor that Induces Cell Cycle Arrest and Apoptosis Via Mitochondrial Pathway. Annals of Surgical Oncology, 21, S711-S720. (SCI, 13/204 Surgery).

• Ruei-Nian Li, Chin-Jen Wu, Zong-Jing Yu, Hsueh-Wei Chang and Shih-Shin Liang (2014, May). Networks development between nicotinic chemical probes and Ca9–22 oral cancer cells by general proteomics analyses. Electrophoresis, 35, 2213–2221. (SCI, 16/76, Chemistry). MOST 100-2113-M-037-012-MY2. 本人為通訊作者.

• Han-Ping Hsu, Chien-Feng Li, Sung-Wei Lee,Wen-Ren Wu, Tzu-Ju Chen, Kwang-Yu Chang, Shih-Shin Liang, Chia-Jung Tsai, Yow-Ling Shiue (2013, Nov). Overexpression of stathmin 1 confers an independent prognostic indicator in nasopharyngeal carcinoma. Tumor Biol., 35, 2619–2629.

• Chao-Jen Kuo, Shih-Shin Liang, Edward Hsi, Shyh-Horng Chiou,Sin-Daw Lin* (2013, Aug). Quantitative proteomics analysis of varicose veins: Identification of a set of differentially expressed proteins related to ATP generation andutilization. Kaohsiung Journal of Medical Sciences, 29(11), 594-605.

• Shih-Shin Liang, Wei-Ting Liao, Chao-Jen Kuo, Chi-Hsien Chou, Chin-Jen Wu and Hui-Min Wang (2013, Jun). Phthalic Acid Chemical Probes Synthesized for Protein-Protein Interaction Analysis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 14, 12914-12930. (SCI, 52/148). NSC 100-2113-M-037-012-MY2. 本人為第一作者、通訊作者.

• Che-Yi Lin, Tien-Szu Pan, Chun-Chan Ting, Shih-Shin Liang, Shu-Hung Huang, Hsiu-Yueh Liu, Edward Cheng-Chuan Ko, Chung-WeiWu, Jen-Yang Tang and Ping-Ho Chen (2013, May). Cytochrome P450 Metabolism of Betel QuidDerived Compounds: Implications for the Development of Prevention Strategies for Oral and Pharyngeal Cancers. The Scientific World Journal, Article ID 618032.

• Shih-Shin Liang, Yow-Ling Shiue, Chao-Jen Kuo, Su-Er Guo, Wei-Ting Liao and Eing-Mei Tsai1 (2013, May). Online Monitoring Oxidative Products and Metabolites of Nicotine by Free Radicals Generation with Fenton Reaction in Tandem Mass Spectrometry. The Scientific World Journal, Article ID 189162. (SCI). NSC 100-2113-M-037-012-MY2. 本人為第一作者、通訊作者.

• Wei-Hsuan Huang, Chao-Neng Tseng, Jen-Yang Tang, Cheng-Hong Yang,Shih- Shin Liang and Hsueh-Wei Chang (2013, Apr). RNA Editing and Drug Discovery for Cancer Therapy. The Scientific World Journal, Article ID 804505.本人為通訊作者.

• Ching-Yu Yen, Shih-Shin Liang, Lo-Yi Han, Han-Lin Chou, Chon-Kit Chou, Shinne-Ren Lin and Chien-Chih Chiu (2013, Mar). Cardiotoxin III Inhibits Proliferation and Migration of Oral Cancer Cells through MAPK and MMP Signaling. The Scientific World Journal, Article ID 650946.

• Yi-Reng Lin, Hin-Kiu Mok, Yuan-Heng Wu, Shih-Shin Liang, Chang-Chun Hsiao, Chun-Hao Huang and Shyh-Horng Chiou (2013, Mar). Comparative proteomics analysis of degenerative eye lenses of nocturnal rice eel and catfish as compared to diurnal zebrafish. MOLECULAR VISION, 19, 623-637.

• Bin Huang, Chien-Hsing Wu, Fu-An Li, Shih-Shin Liang , Ying-Hua Shieh, Ling Danny Wang (2013, Jan). Rosuvastatin-regulated post-translational phosphoproteome in human umbilical vein endothelial cells. KAOHSIUNG JOURNAL OF MEDICAL SCIENCES, 29, 347-352.

• Wei-Ting Liao, Tsi-Shu Huang, Chien-Chih Chiu, Jian-Liang Pan, Shih-Shin Liang, Bing-Hung Chen, Shi-Hui Chen, Po-Len Liu, Hui-Chun Wang, Zhi-Hong Wen, Hui-Min Wang and Shu-Wen Hsiao (2012, May). Biological Properties of Acidic Cosmetic Water from Seawater. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 13, 5952-5971.

• Liang, Shih-Shin, Chen, S. L., and Chen, S. H.* (2011, Jun). Diverse macroporous spheres synthesized by multiple emulsion polymerization for online integrated protein analyses. Chem. Commun., 47. P.8385-P.8387. (SCI).

專書

• Authors: Thomas Shyh-Horng Chiou, Min-Yi Lee, Chao-Jen Kuo and Shih-Shin Liang. Recent Advances in Proteomics Research (ISBN:978-1-62948-218-7) Chapter five Title: Application of Quantitative Proteomics to Disease Diagnosis and Prognosis Using Stable Isotope Dimethyl Labeling Coupled with Shotgun Proteomics Strategy and Multiple Reaction Monitoring pp.119-137 (ISBN: 978-1-62948-218-7). New York, USA: NOVA. Jun, 2013.

•    學生研究成果

• 學生	研究成果
  BT106張育慈	以還原胺化法修飾乙型受體素並使用液相層析串聯式質譜儀定量分析 研究生：張育慈 指導教授：梁世欣     乙型受體素俗稱瘦肉精，其作用類似於腎上腺素。萊克多巴胺早期被用於治療氣喘，但對人體的毒性太大，在臨床用藥上被淘汰，在1999年，美國FDA核准此類藥物添加在動物飼料中，使動物體內脂肪降解，促進蛋白質生成，而大幅降低飼養成本，增加經濟效益，不過藥物殘留則會造成食安問題。當瘦肉精殘留濃度過高時，可能會引起急性中毒，對於患有心、肝、腎臟慢性病患者而言，食用風險較健康者大。因此，檢測瘦肉精殘留含量是必要且重要的。此篇論文係利用還原胺化法將瘦肉精做化學修飾，並用於檢測方法開發，且檢測儀器為食品安全中最常被使用的分析工具—液相層析串聯式質譜儀，做定性與定量分析。