實驗室招生影片

 

聯絡方式:

• 辦公室: 第一教學大樓N1029室
• 實驗室: 第一教學大樓N1024室
• 電話: (07) 3121101 ext. 2369
• 傳真: (07) 3125339
• 電子郵件: jclee@kmu.edu.tw

學經歷:

• 中國文化大學生物系學士(1991)
• 中國文化大學生物科技研究所碩士(1993)
• 國防醫學院生命科學研究所理學博士(1998)
• 中央研究院 分子生物研究所 博士後研究 (1998-1999)
• 國家衛生研究院 生物技術與藥物研究組 博士後研究 (1999-2003)
• 工業技術研究院 生物醫學工程中心 研究員 (2003-2004)
• 高雄醫學大學 生物科技學系 助理教授 (2004-2011)
• 高雄醫學大學 生物科技學系 副教授 (2011-2014)
• 高雄醫學大學 物科技學系 教授 (2014-present)

 

獎項/榮譽

• 101學年度 高雄醫學大學 教學優良教師
• 科技部生命科學發展司「生物學之生化及分子生物」學門之研究計劃複審委員 (102-103年度)

主負責授課科目:

• 病毒學
• 病毒腫瘤學
• 分子細胞生物學
• 遺傳工程學

 動物實驗教學影片

 

研究興趣:

• 針對C型肝炎病毒及登革熱病毒進行抗病毒之天然藥物及小分子開發及藥物機轉研究。
• 病毒複製機轉研究

 

研究計畫：

• 抗氧化分子與登革病毒複製之關連性探討及抗病毒藥物開發 (科技部 MOST-104-2320-B-037-025-MY3)
• 探索登革病毒複製之細胞因子與抗病毒標靶藥物開發 (奇美/高醫計畫 104CM-KMU-05)
• 探討萃取自海洋海綿及真菌之三帖類似物於抗病毒及抗病毒性發炎的活性分析與機制探討(中山/高醫 NSYSU-KMU 104-I011)
• 菟葵萃取物抗病毒及抗發炎之活性成分分析 (科技部 MOST 103-2622-B03-CC3)
• 以台灣產原生植物及索羅門群島植物之成分與小分子合成開發抗登革及C型肝炎病毒之新穎性藥物 (高醫大 KMU-TP103H12)
• 第一型血紅素氧化(heme oxygenase-1)與C型肝炎病毒複製及病毒性致癌的關連性探討(科技部NSC101-2311-B-037-002-MY3)

 

科技部大專學生研究計畫：

• 104年度：Mi-RNA let7c 藉由誘導血紅素氧化酶抑制HCV病毒複製 ( 鄭雅文  104-2815-C-037-025-B)
• 102年度: 建構高通量篩選抗登革病毒聚合酶(RNA-dependent RNA polymerase)抑制劑之平台 (吳依亭 102-2815-C-037-012-B)
• 101年度：探討Sulfpraphane及其衍生物之抗C型肝炎病毒複製及對病毒性發炎抑制機轉之研究 (吳依亭 101-2815-C-037-025-B)
• 99年度：以昆蟲桿狀病毒AcMNPV之融合包涵體快速製備抗體 (李婉華 99-2815-C-037-023-B)
• 98年度：探討黃芩萃取物抑制C型肝炎複製之作用機制 (吳宇軒 98-2815-C-037-028-B)
• 97年度：以桿狀病毒持續感染之昆蟲細胞分析polh啟動子在細胞的表現及作為高效能蛋白質表達系統的評估 (林昱伶 97-2815-C-037-006-B)
• 97年度：以昆蟲桿狀病毒作C型肝炎假病毒顆粒建立篩選抑制C型肝炎病毒入侵細胞之藥物平台 (林俊光 97-2815-C-037-005-B)
• 96年度：抗干擾素治療之C型肝炎病毒基因體分析及其複製子系統之建立 (陳芳君 96-2815-C-037-024-B)
• 96年度：建構誘發式的基因剔除系統及RNAi干擾系統作為昆蟲桿狀病毒功能性基因分析平台：以AcMNPV p35基因為分析模式 (黃怡嘉 96-2815-C-037-025-B)
• 95年度：腸病毒71型protease和RNA dependent RNA polymerase活性檢測系統之開發 (陳郁芬 95-2815-C-037-007-B)
• 94年度：高速篩選C型肝炎病毒藥物系統的開發 (曾敬凱 94-2815-C-037-015-B)
• 94年度：建構腸病毒71型的replicon序列 (陳郁芬 94-2815-C-037-006-B)

 

期刊論文：

2015:

• Jin-Ching Lee* , Chin-Kai Tseng, Yu-Hsuan Wua, Neerja Kaushik-Basu, Chun-Kuang Lin, Wei-Chun Chen, Huey-Nan Wu. (2015)Characterization of the activity of 2-C-methylcytidine against dengue virus replication. Antiviral Research. 116. 1-9. (IF=3.434; 58/254 =22.83%)
• Chun-Kuang Lin, Chin-Kai Tseng, Kai-Hsun Chen, Shih-Hsiung Wu,Chih-Chuang Liaw*, Jin-Ching Lee*.(2015)Betulinic acid exerts anti-hepatitis C virus activity via the suppression of NF-κB and MAPK-ERK1/2-mediated cyclooxygenase-2 expression. British Journal of Pharmacology (in press). (IF=5.06; 21/260 =8.07%)
• Wei-Chun Chen, Chin-Kai Tseng, Yen-Hsu Chen, Chun-Kuang Lin, Shih-hsien Hsu, Shen-Nien Wang, Jin-Ching Lee* . (2015)HCV NS5A up-regulates COX-2 expression via IL-8-mediated activation of the ERK/JNK MAPK pathway. PLoS ONE. 10(7): e0133264. (IF=3.7; 7/56 =12.5%)

2014:

• Jin-Ching Lee* , Chin-Kai Tseng, Kung-Chia Young, Hung-Yu Sun, Shainn-Wei Wang, Wei-Chun Chen, Chun-Kuang Lin, Yu-Hsuan Wu. (2014) Andrographolide exerts anti-hepatitis C virus activity by upregulating heme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells. British Journal of Pharmacology 171 237-252. (IF=5.06; 21/260 =8.07%)
• Chih-Hua Tseng^*, Chun-Kuang Lin, Yeh-Long Chen³, Chih-Yao Hsu, Huey-Nan Wu, Chin-Kai Tseng, and Jin-Ching Lee * (2014) Synthesis, Antiproliferative and Anti-Dengue Virus Evaluations of 2-Aroyl-3-arylquinoline Derivatives. European Journal of Medicinal Chemistry. In press. (IF=3.49; 13/59 =22%)
• Chin-Kai Tseng, Chun-Kuang Lin, Hsueh-Wei Chang, Yu-Hsuan Wu, Feng-Lin Yen, Fang-Rong Chang, Wei-Chun Chen, Chi-Chen Yeh, Jin-Ching Lee^* (2014)Aqueous Extract of Gracilaria tenuistipitata Suppresses LPS-Induced NF-kB and MAPK Activation in RAW 264.7 and Rat Peritoneal Macrophages and Exerts Hepatoprotective Effects on Carbon Tetrachloride- Treated Rat. PLoS ONE 9(1): e86557. doi: 10.1371/journal.pone.0086557. (IF=3.7; 7/56 =12.5%)
• Chun-Chieh Lin, Peiju Tsai, Hung-Yu Sun, Mei-Chi Hsu, Jin-Ching Lee , I-Chin Wu, Chiung-Wen Tsao, Ting-Tsung Chang, Kung-Chia Young*.(2014)Apolipoprotein J, a glucose up-regulated molecular chaperone stabilizes core and NS5A to promote infectious hepatitis C virus virion production. Journal of Hepatology. 61(5), 984-993
• Yuan-Bin Cheng,Yi-Ting Chien,Jin-Ching Lee , Chin-Kai Tseng, Hui-ChunWang, I-Wen Lo, Yi-Hung Wu,Sheng-Yang Wang,Yang-Chang Wu,and Fang-Rong Chang. (2014)Limonoids from the Seeds of Swietenia macrophylla with Inhibitory Activity against Dengue Virus 2. Journal of Natural of Products.95. 2155-2165. (IF=3.947; 21/199 =10.5%)
• Peng-Yeh Lai, Chia-Tse Hsu, Shao-Hung Wang, Jin-Ching Lee, Min-Jen Tseng, Jaulang Hwang, Wen-Tsai Ji and Hau-Ren Chen. (2014). Production of a neutralizing antibody against envelope protein of dengue virus 2 type 2 using linear array epitope (LAE) technique. Journal of General Virology. 95, 2155-2165 I.F.=3.127; 44/160 (27.5%)
• I-Ling Lin, Han-Lin Chou, Jin-Ching Lee , Feng-Wei Chen, Yao Fong, Wei-Chiao Chang, Hurng Wern Huang, Chang-Yi Wu, Wen-Tsan Chang, Hui-Min David Wang and Chien-Chih Chiu* (2014)The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB. Cancer Cell International. 14:1-7

2013:

• Wei-Chun Chen, Sheng-Yang Wang, Chien-Chih Chiu, Chin-Kai Tseng, Chun-Kuang Lin, Hui-Chun Wang and Jin-Ching Lee* . (2013)Lucidone Suppresses Hepatitis C Virus Replication by Nrf2-Mediated Heme Oxygenase-1 Induction. Antimicrobial Agents and Chemotherapy.57(3):1180-1191. (IF=4.5; 31/260 =11.9%)
• Kuan-Jen Chen., Chin-Kai Tseng, Fang-Rong Chang, Jin-Iong Yang, Chi-Chen Yeh, Wei-Chun Chen, Shou-Fang Wu, Hsueh-Wei Chang, Jin-Ching Lee* . (2013)Aqueous Extract of the Edible Gracilaria tenuistipitata Inhibits Hepatitis C Viral Replication via Cyclooxygenase- 2 Suppression and Reduces Virus-Induced Inflammation. PLoS ONE. 8(2): e57704. doi:10.1371/journal.pone.0057704. (IF=3.7; 7/56 =12.5%)
• Ying-Ting Lin, Yu-Hsuan Wu, Chin-Kai Tseng, Chun-Kuang Lin, Wei-Chun Chen, Yao-Chin Hsu, Jin-Ching Lee* . (2013)Green Tea Phenolic Epicatechins Inhibit Hepatitis C Virus Replication via Cycloxygenase-2 and Attenuate Virus-Induced Inflammation. PLoS ONE. 8(1): e54466. doi:10.1371/journal.pone.0054466. (IF=3.7; 7/56 =12.5%)
• Huang-Kai Peng, Wei-Chun Chen, Ying-Ting Lin, Chin-Kai Tseng, Shiang-Yu Yang, Cherng-Chyi Tzeng, Jin-Ching Lee* , Shyh-Chyun Yang*. (2013) Anti-hepatitis C virus RdRp activity and replication of novel anilinobenzothiazole derivatives. Antiviral Research 100: 269-275. (IF=3.92; 45/260 =17.3%)
• Hung-Yu Sun, Chun-Chieh Lin, Jin-Ching Lee , Shainn-Wei Wang, Pin-Nan Cheng, I-Chin Wu, Ting-Tsung Chang, Ming-Derg Lai, Dar-Bin Shieh, Kung-Chia Young. (2013) Very low-density lipoprotein/lipo-viro particles reverse lipoprotein lipase-mediated inhibition of hepatitis C virus infection via apolipoprotein C-III. Gut. 62:1193–1203. (IF=10.732; 3/74 =4%)
• Huang-Kai Peng, Wei-Chun Chen, Jin-Ching Lee , Shiang-Yu Yang, Cherng-Chyi Tzeng, Ying-Ting Lin and Shyh-Chyun Yang. (2013)Novel anilinocoumarin derivatives as agents against hepatitis C virus by the induction of IFN-mediated antiviral responses. Organic & Biomolecular Chemistry. 11, 1858-1866. (IF=3.568; 11/55 =20%)
• Jen-Yang Tang, Jin-Ching Lee , Ming-Feng Hou,Chun-LinWang, Chien-Chi Chen, Hurng-Wern Huang, and Hsueh-Wei Chang. (2013)Alternative Splicing for Diseases, Cancers, Drugs, and Databases.Scientific World Journal vol 2013, Article ID 703568, 8 pages, doi: 10.1155/2013/703568. (IF=1.73; 13/56 =23.2%)
• Jin-Ching Lee , Ming-Feng Hou, Hurng-Wern Huang, Fang-Rong Chang, Chi-Chen Yeh, Jen-Yang Tang and Hsueh-Wei Chang. (2013)Marine algal natural products with anti-oxidative, anti-inflammatory, and anti-cancer properties. Cancer Cell International. 13:55.(IF=2.09; 121/196 =61.7%)
• Guan-Ting Liu, Chi-Shin Hwang, Chia-Hung Hsieh, Chih-Hao Lu, Sunny Li-Yun Chang, Jin-Ching Lee , Chien-Fu Huang, and Hao-Teng Chang. (2013) Eosinophil-Derived Neurotoxin Is Elevated in Patients with Amyotrophic Lateral Sclerosis. Mediators of Inflammation. ID 421389, 7 pages. doi: 10.1155/2013/421389. (IF=3.882; 39/135 =28.8%)

2012:

• Shou-Fang Wu, Chun-kuang Lin, Yen-Shu Chuang, Fang-Rong Chang, Chin-Kai Tseng, Yang-Chang Wu, and Jin-Ching Lee* (2012) Anti-Hepatitis C Virus Activity of 3-hydroxy caruilignan C from Swietenia macrophylla Stems. Journal of Viral Hepatitis 19, 364-370(IF=3.502; 19/72 =26%)
• Huang-Kai Peng, Chun-Kuang Lin, Chin-Kai Tseng, Cherng-Chyi Tzeng, Jin-Ching Lee* , and Shyh-Chyun Yang*. (2012) Synthesis and anti-HCV activity evaluation of anilinoquinoline derivatives. Bioorganic and Medicinal Chemistry Letters .22, 1107-1110. *corresponding author (IF=2.66; 19/56 =33.9%)
• Daniel B. Nichols, Guy Fournet, K.R. Gurukumar, Amartya Basu, Jin-Ching Lee , Naoya Sakamoto, Frank Kozielski, Ira Musmuca, Benoît Joseph, Rino Ragno, Neerja Kaushik-Basu. (2012). Inhibition of hepatitis C virus NS5B polymerase by S-trityl-L-cystein derivatives. European Journal of Medicinal Chemistry. 1-9.(IF=3.499; 13/59 =22%)
• Andriy G. Golub, K.R. Gurukumar, Amartya Basu, Volodymyr G. Bdzhola, Yaroslav Bilokin, Sergiy M. Yarmoluk, Jin-Ching Lee , Tanaji T. Talele, Daniel B. Nichols, Neerja Kaushik-Basu. (2012)Discovery of new scaffolds for rational design of HCV NS5B polymerase inhibitors. European Journal of Medicinal Chemistry. 58: 258-264. (IF=3.499; 13/59 =22%)
• Chi-Chen Yeh, Jing-Iong Yang, Jin-Ching Lee , Chao-Neng Tseng, Ya-Ching Chan, You-Cheng Hseu, Jen-Yang Tang, Li-Yeh Chuang, Hurng-Wern Huang, Fang-Rong Chang* and Hsueh-Wei Chang*. (2012)Anti-proliferative effect of methanolic extract of Gracilaria tenuistipitata on oral cancer cells involves apoptosis, DNA damage, and oxidativestress. BMC Complementary & Alternative Medicine. 12:142. (IF=2.082; 7/21 =33.3%)
• Jing-iong Yang, Chi-chen Yeh, Jin-Ching Lee , Szu-cheng Yi, Chao-neng Tseng, Hsueh-wei Chang , Jing-iong Yang, Chi-chen Yeh, Jin-ching Lee, Szu-cheng Yi, Chao-neng Tseng, Hsueh-wei Chang. (2012)Aqueous Extracts of the Edible Gracilaria tenuistipitata Are Protective Against H2O2-Induced DNA Damage, Growth Inhibition, and Cell Cycle Arrest. Molecules. 17, 7241-7254. (IF=2.428; 23/55 =41.8%)

2011:

• Chin-kai Tseng, Kuan-Jen Chen, Chun-Kuang Lin, Shih-hsien Hsu, Jin-Ching Lee * (2011) An in vitro Coupled Transcription/Translation Reporter System for Hepatitis C Virus RNA-dependent RNA Polymerase. Analytical Biochemistry 418, 50-57. (IF=3.236; 16/73 =21.9%)
• Jin-Ching Lee,* Chen WC, Wu SF, Tseng CK, Chiou CY, Chang FR, Hsu SH, Wu YC.(2011). Anti-hepatitis C virus activity of Acacia confusa extract via suppressing cyclooxygenase-2. Antiviral Research. 89, 35-42(IF=4.439; 34/252 =13.4%)
• Jin-Ching Lee,* C.-K. Tseng, S.-F. Wu, F.-R. Chang, C.-C. Chiu and Y.-C. Wu. (2011).San-Huang-Xie-Xin-Tang extract suppresses hepatitis C virus replication and virus-induced cyclooxygenase-2 expression. Journal of Viral Hepatitis 18, e315-e324  (IF=3.502; 19/72 =26%)
• Ying-Ting Lin, Kuo-Jung Huang, Chin-Kai Tseng, Kuan-Jen Chen, Hui-Min Wang, and Jin-Ching Lee* (2011). Efficient In Silico Assay of Inhibitors of Hepatitis C Virus RNA-Dpendent RNA Polymerase by Structure-Based Virtual Screening and In Vitro Evaluation. ASSAY and Drug Development Technologies.(DOI: 10.1089/adt.2010.0341)(IF=2.713; 25/67 =37%)

研討會論文

• Wannhsin chen,Jun-Jae Haung,Kwang-Ning Chang, Hui-Chu Hsieh, Jin-Ching Lee, Lih-Tao Hsu, Mei-Ju Yang, Li Chun Pan, Chih-Ching Chien, Ping-Fu Cheng, Chih-Yen Tasi, Yi-Ting Chen, Tzu-Ling Tseng, Chao-Ying Kuo. Proteomics research for mouse and human embryonic stem cells. 2004. 3rd International Proteomics Conference (IPC’03), 1st Proteomics Conference Joint Meeeting of AOHUPO 2004.
  Wannhsin Chen, Szu-Yu Lin, Jin-Ching Lee, Shiaw-Min Hwang, Chii-Ming Lee, Yao-Horng Wang and Chao-Ying Kuo. In vitro myotube formation of ascorbic acid reacted porcine mesenchymal stem cells. 2006. 4th Internal Society for Stem Cell Research (ISSCR) Annual Meeting, . 
• Chin-kai, Tseng , Yang-Chang Wu , Chun-Kuang Lin , Fang-Rong Chang & Jin-Ching Lee*. Inhibition of hepatitis c virus replication by catechin extracted from Chinese and Herbal Medicine. 2007 10th Southeast Asian Western Pacific Regional Meeting of Pharmacologists and the 41st Annual Scientific Meeting of ASCEPT, .
• Wei-Chun Chen, Chin-kai Tseng, Yu-Fen Chen, Jin-Ching Lee*. Establishment of hepatitis C virus-pseudotyped baculovirus for screening inhibitors against viral entry .BIT’s 1st Annual World Summit of Antivirals (WSA-2008). July 20-22, 2008 in Kunming.
• Chin-kai Tseng, Chun-Kuang Lin, Jin-Ching Lee*. A rapid reporter assay for drug screening of anti-hepatitis C virus RNA-dependent RNA polymerase. 2009 IBC’s 4th Annual Drug Discovery & Development JAPAN conference
  Jin-Ching Lee* Anti-Hepatitis C Virus Activity of Acacia confusae Extract via Suppressing the Cyclooxygenase. 2BIT’s 1st World Congress of Virus and Infections (WCVI) which will be held during July 31~August 3, 2010 in Busan, Republic of Korea.

專利：

1. 用於抑制癌細胞生長、誘導癌細胞細胞凋亡及/或造成DNA傷害之藥物組成物，以及紅藻龍需菜醇類萃取物的用途 （中華民國 I458486; 2014.11.01~2033.02.26）

2. 用於減少自由機誘導之細胞之DNA損傷的試劑、藥物或保健食品、與皮膚保養或化妝品，以及紅藻龍需菜萃取物的用途 （中華民國 I492766; 201507.21~2031.01.20）