李景欽教授 (Jin-Ching Lee)
個人簡介
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研究介紹
C型肝炎病毒(hepatitis C virus; HCV)之研究及抗病毒天然物開發
C型肝炎病毒(Hepatitis C Virus; HCV)是造成慢性肝炎、肝癌的原因之一，除了干擾素治療外，目前已有數個針對病毒蛋白的標靶藥物用於臨床治療，但由於HCV為RNA病毒，其基因的高突變率，將提高抗藥性的產生，因此，繼續開發抗HCV輔助藥物或輔助食品有其必要性。在天然藥物開發方面，主要以中草藥、植物或食品為主，配合以活性為導向的萃取策略，在HCV的研究策略與成果簡述如下：
1. 建立篩選抗HCV NS5B聚合酶藥物之平台：
構築一個雙報導基因質體p(+)FLuc-(–)UTR-RLuc，經過細胞的基因轉殖及高敏感性的冷光測定，能正確地同時反應抑制劑對細胞的毒性與對RdRp活性的影響，可以排除因細胞毒性所引起的抗病毒活性，避免篩選出偽陽性的抑制藥物，提高藥物篩選效率。可藉由隨機式或以蛋白質結構為依據進行電腦模擬小分子藥物篩選。
2. 以細胞蛋白為標靶之藥物開發與機轉研究：
藉由藥物基因體學的研究發現，抑制細胞中還氧合酶-2(cyclooxygenase-2；COX-2)表現或增強第一型血紅素氧化酶 (haeme oxygenase-1，HO-1)表現量可以達到抗病毒效果，證實細胞內COX-2及HO-1訊息傳遞路徑可作為抑制HCV複製的藥物標靶，由於細胞基因的突變率遠低於RNA病毒基因的突變率，相較於以病毒基因產物為標靶的藥物開發，我們的研究成果提供一個可以有效解決抗藥性的藥物研發方向，目前已有數個抗病毒天然藥物發表。未來亦將往粒腺體對病毒複製與肝癌化的關係研究。
3. 病毒蛋白對細胞發炎因子的影響：
研究發現病毒感染將透過interleukin-8所調控的JNK及ERK訊息傳遞路徑影響發炎因子COX-2的誘發，而所誘發的COX-2又可以正向促進病毒複製，此週而復始終將導致肝疾病產生。
登革病毒(Dengue virus; DENV)之研究及抗病毒天然藥物開發
登革病毒是一種藉由蚊子傳播的病毒，主要流行於熱帶及亞熱帶地區，其威脅為引起登革熱(Dengue fever)、登革出血熱(Dengue haemorrhagic fever)甚至是登革休克症候群(Dengue shock syndrome)。目前尚未有治療登革熱的藥物也沒有完全有效的疫苗，因此開發新穎天然物成為抗病毒之輔助/保健食品或藥物為值得研究的方向。除了分析病毒蛋白酵素活性，利用次世代定序(Nest Generation Sequencing; NGS)分析DENV感染對宿主細胞基因表現的影響，進而找出病毒複製的關鍵因子以及找出有潛力的抗病毒策略。成果簡述如下：
1. 建立篩選抗DENV NS5聚合酶藥物之平台：
構築一個雙報導基因質體p(+)RLuc-(–)DV-UTRΔC-FLuc，經過細胞的基因轉殖及高敏感性的冷光測定，能正確地同時反應抑制劑對細胞的毒性與對RdRp活性的影響。
2. 細胞因子、微型核糖核酸與病毒複製之研究
透過次世代定序的結果分析發現第一型血紅素氧化酶 (haeme oxygenase-1; HO-1)與病毒複製有關，且證實誘導HO-1訊息傳遞路徑可作為抑制登革病毒複製的藥物標靶。目前則發現病毒感染所影響的粒線體能量生成會影響下游脂質代謝，此現象有利於登革病毒蛋白質生成及RNA複製，此外，也發現數個微型核糖核酸(MicroRNA)與登革病毒複製有關，這些新穎性的發現將是未來的研究方向。
研究計畫
研究計畫：
- 開發抗登革病毒感染及登革出血熱之新穎性小分子及天然藥物 (高醫 KMU-TP104H03)
- 一種水果萃取物作為抗發炎及保肝之保健食品研發 (高醫 KMU-PT104006)
- 抗氧化分子與登革病毒複製之關連性探討及抗病毒藥物開發 (科技部 MOST-104-2320-B-037-025-MY3)
- 探索登革病毒複製之細胞因子與抗病毒標靶藥物開發 (奇美/高醫計畫 104CM-KMU-05)
- 探討萃取自海洋海綿及真菌之三帖類似物於抗病毒及抗病毒性發炎的活性分析與機制探討(中山/高醫 NSYSU-KMU 104-I011)
- 菟葵萃取物抗病毒及抗發炎之活性成分分析 (科技部 MOST 103-2622-B03-CC3)
- 以台灣產原生植物及索羅門群島植物之成分與小分子合成開發抗登革及C型肝炎病毒之新穎性藥物 (高醫大 KMU-TP103H12)
- 第一型血紅素氧化(heme oxygenase-1)與C型肝炎病毒複製及病毒性致癌的關連性探討(科技部NSC101-2311-B-037-002-MY3)
科技部大專學生研究計畫：
- 105年度：探討compound G抑制登革病毒作用機轉（魏旨谷 105-2815-C-037-034-B)
- 105年度：探討中草藥萃取物compound S抑制登革病毒的分子機轉（蔡嘉瑩 105-2815-C-037-042-B)
- 104年度：Mi-RNA let7c 藉由誘導血紅素氧化酶抑制HCV病毒複製 ( 鄭雅文 104-2815-C-037-025-B)
- 102年度: 建構高通量篩選抗登革病毒聚合酶(RNA-dependent RNA polymerase)抑制劑之平台 (吳依亭 102-2815-C-037-012-B)
- 101年度：探討Sulfpraphane及其衍生物之抗C型肝炎病毒複製及對病毒性發炎抑制機轉之研究 (吳依亭 101-2815-C-037-025-B)
- 99年度：以昆蟲桿狀病毒AcMNPV之融合包涵體快速製備抗體 (李婉華 99-2815-C-037-023-B)
- 98年度：探討黃芩萃取物抑制C型肝炎複製之作用機制 (吳宇軒 98-2815-C-037-028-B)
- 97年度：以桿狀病毒持續感染之昆蟲細胞分析polh啟動子在細胞的表現及作為高效能蛋白質表達系統的評估 (林昱伶 97-2815-C-037-006-B)
- 97年度：以昆蟲桿狀病毒作C型肝炎假病毒顆粒建立篩選抑制C型肝炎病毒入侵細胞之藥物平台 (林俊光 97-2815-C-037-005-B)
- 96年度：抗干擾素治療之C型肝炎病毒基因體分析及其複製子系統之建立 (陳芳君 96-2815-C-037-024-B)
- 96年度：建構誘發式的基因剔除系統及RNAi干擾系統作為昆蟲桿狀病毒功能性基因分析平台：以AcMNPV p35基因為分析模式 (黃怡嘉 96-2815-C-037-025-B)
- 95年度：腸病毒71型protease和RNA dependent RNA polymerase活性檢測系統之開發 (陳郁芬 95-2815-C-037-007-B)
- 94年度：高速篩選C型肝炎病毒藥物系統的開發 (曾敬凱 94-2815-C-037-015-B)
- 94年度：建構腸病毒71型的replicon序列 (陳郁芬 94-2815-C-037-006-B)
期刊論文
期刊論文：
2018:
- Chih-Hua Tseng, Chun-Kuang Lin, Yeh-Long Chen, Chin-Kai Tseng, Jar-Yu Lee , Jin-Ching Lee* (2018) Discovery of naphtho[1,2-d]oxazole derivatives as potential anti-HCV agents through inducing heme oxygenase-1 expression. European Journal of Medicinal Chemistry 143:970-982 (IF=4.519; 4/60=6.66%)
2017:
- Jung-Sheng Yu, Chin-Kai Tseng, Chun-Kuang Lin, Yao-Chin Hsu, Yu-Hsuan Wu, Ching-Liang Hsieh and Jin-Ching Lee*. (2017) Celastrol inhibits dengue virus replication via up-regulating type I interferon and downstream interferon-stimulated responses. Antiviral Research.137:49-57. (IF=4.271; 38/257=14.785%)
- Hung-Yu Sun, Chun-Chieh Lin, Pei-Ju Tsai, Wei-Jen Tsai, Jin-Ching Lee, Chiung-Wen Tsao, Pin-Nan Cheng, I-Chin Wu, Yen-Cheng Chiu, Ting-Tsung Chang and Kung-Chia Young. (2017) Lipoprotein lipase liberates free fatty acids to inhibit HCV infection and prevent hepatic lipid accumulation. Cellular Microbiology. 19(4). (IF=4.554; 22/125=17.6%)
- Jung-Sheng Yu, Yu-Hsuan Wu, Chin-Kai Tseng, Chun-Kuang Lin, Yao-Chin Hsu, Yen-Hsu Chen* and Jin-Ching Lee*. (2017) Schisandrin A inhibits dengue viral replication via upregulating antiviral interferon responses through STAT signaling pathway. Scientific Reports. 7:45171. (IF=4.259; 10/64 =15.625%)
- Chun-Kuang Lin, Chin-Kai Tseng, Yu-Hsuan Wu, Chih-Chuang Liaw, Chun-Yu Lin,Chung-Hao Huang, Yen-Hsu Chen and Jin-Ching Lee*. (2017) Cyclooxygenase-2 facilitates dengue virus replication and serves as a potential target for developing antiviral agents.Scientific Reports, 7:44701. (IF=4.259; 10/64 =15.625%)
- Peiju Tsai, Chun-Chieh Lin, Hung-Yu Sun, Jin-Ching Lee, Ting-Tsung Chang, Kung-Chia Young. (2017) Viral dynamics of persistent hepatitis C virus infection in high-sensitive reporter cells resemble patient's viremia. Journal of Microbiology, Immunology and Infection. pii: S1684-1182(17)30058-0. (IF=2.97; 35/84 =41.66%)
- Sveva Pelliccia, Yu-Hsuan Wu, Antonio Coluccia, Giuseppe La Regina, Chin-Kai Tseng, Valeria Famiglini, Domiziana Masci, John Hiscott, Jin-Ching Lee*, Romano Silvestri*(2017) Inhibition of Dengue Virus Replication by Novel Inhibitors of RNA-dependent RNA Polymerase and Protease Activities.Journal of Enzyme Inhibition and Medicinal Chemistry ;32(1):1091-1101. (IF=4.293; 5/60 =8.33%)
- Chin-Kai Tseng, Sung-Po Hsu, Chun-Kuang Lin, Yu-Hsuan Wu, Jin-Ching Lee*, Kung-Chia Young*. (2017) Celastrol inhibits hepatitis C virus replication by upregulating heme oxygenase-1 via the JNK MAPK/Nrf2 pathway in human hepatoma cells. Antiviral Research, 146:191-200. (IF=4.271; 38/257=14.785%)
- Jin-ChingLee, Chin-KaiTseng, Chun-KuangLin, Chih-HuaTseng. (2017) Discovery of novel diarylpyrazolylquinoline derivatives as potent anti-dengue virus agents. European Journal of Medicinal Chemistry,141:282-292. (IF=4.519; 4/60=6.66%)
2016:
- Jung-Sheng Yu, Wei-Chun Chen, Chin-Kai Tseng, Chun-Kuang Lin, Yao-Chin Hsu, Yen-Hsu Chen*, Jin-Ching Lee*.(2016) Sulforaphane Suppresses Hepatitis C Virus Replication by Up-Regulating Heme Oxygenase-1 Expression through PI3K/Nrf2 Pathway. PLoS ONE. 11(3): e0152236. doi:10.1371/journal.pone.0152236. (IF=3.057; 11/63 =17.46%)
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Chin-Kai Tseng, Chun-Kuang Lin, Yu-Hsuan Wu, Yen-Hsu Chen, Wei-Chun Chen, Kung-Chia Young, Jin-Ching Lee*. (2016)Human heme oxygenase 1 is a potential host cell factor against dengue virus replication. Scientific Reports. 6:32176. doi: 10.1038/srep32176. (IF=5.228; 7/63 =11.111%)
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Jin-Ching Lee, Fang-Rong Chang, Shu-Rong Chen, Yu-Hsuan Wu, Hao-Chun Hu, Yang-Chang Wu, Anders Backlund, Yuan-Bin Cheng*. (2016) Anti-Dengue Virus Constituents from Formosan Zoanthid Palythoa mutuki. Marine Drugs. 14(8), 151. doi:10.3390/md14080151. (IF=3.345; 13/59 =22.033%)
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Chun-Kuang Lin, Yu-TingWang, Er-Mao Hung, Yu-Liang Yang, Jin-Ching Lee, Jyh-horng Sheu, Chih-Chuang Liaw. (2016)Butyrolactones and Diketopiperazines from Marine Microbes: Inhibition Effects on Dengue Virus Type 2 Replication. Planta Medica. doi:10.1055/s-0042-112998. (IF=1.990; 70/209 =34.49%)
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Yuan-Bin Cheng, Jin-Ching Lee, I-Wen Lo, Shu-Rong Chen, Hao-Chun Hu, Yu-Hsuan Wu, Yang-Chang Wu*, Fang-Rong Chang*.(2016)Ecdysones from Zoanthus spp. with inhibitory activity against dengue virus 2.Bioorganic and Medicinal Chemistry Letters. 9;26(9):2344-8. (IF=2.486; 23/59 =38.98%)
- Wei-Chun Chen, Chin-Kai Tseng, Bing-Hung Chen, Chun-Kuang Lin and Jin-Ching Lee*. (2016)Grape Seed Extract Attenuates Hepatitis C Virus Replication and Virus-Induced Inflammation. Frontiers in Pharmacology. 7:490. (IF=4.418; 33/255=12.941%)
2015:
- Jin-Ching Lee*, Chin-Kai Tseng, Yu-Hsuan Wua, Neerja Kaushik-Basu, Chun-Kuang Lin, Wei-Chun Chen, Huey-Nan Wu. (2015)Characterization of the activity of 2-C-methylcytidine against dengue virus replication. Antiviral Research. 116. 1-9. (IF=3.434; 58/254 =22.83%)
- Chun-Kuang Lin, Chin-Kai Tseng, Kai-Hsun Chen, Shih-Hsiung Wu,Chih-Chuang Liaw*, Jin-Ching Lee*.(2015)Betulinic acid exerts anti-hepatitis C virus activity via the suppression of NF-κB and MAPK-ERK1/2-mediated cyclooxygenase-2 expression. British Journal of Pharmacology. 172: 4481-4492 (IF=5.06; 21/260 =8.07%)
- Wei-Chun Chen, Chin-Kai Tseng, Yen-Hsu Chen, Chun-Kuang Lin, Shih-hsien Hsu, Shen-Nien Wang, Jin-Ching Lee* . (2015)HCV NS5A up-regulates COX-2 expression via IL-8-mediated activation of the ERK/JNK MAPK pathway. PLoS ONE. 10(7): e0133264. (IF=3.7; 7/56 =12.5%)
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Chih-Chuang Liaw,Yu-Liang Yang, Chun-Kuang Lin, Jin-Ching Lee, Wen-Ying Liao, Chia-Ning Shen, Jyh-Horng Sheu, and Shih-Hsiung Wu. (2015) New Meroterpenoids from Aspergillus terreus with Inhibition of Cyclooxygenase‑2 Expression. Organic Letters 17, 2330-2333. (IF=6.636; 4/57=7.01%)
2014:
- Jin-Ching Lee* , Chin-Kai Tseng, Kung-Chia Young, Hung-Yu Sun, Shainn-Wei Wang, Wei-Chun Chen, Chun-Kuang Lin, Yu-Hsuan Wu. (2014) Andrographolide exerts anti-hepatitis C virus activity by upregulating heme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells. British Journal of Pharmacology 171 237-252. (IF=5.06; 21/260 =8.07%)
- Chih-Hua Tseng*, Chun-Kuang Lin, Yeh-Long Chen, Chih-Yao Hsu, Huey-Nan Wu, Chin-Kai Tseng, and Jin-Ching Lee* (2014) Synthesis, Antiproliferative and Anti-Dengue Virus Evaluations of 2-Aroyl-3-arylquinoline Derivatives. European Journal of Medicinal Chemistry. In press. (IF=3.49; 13/59 =22%)
- Chin-Kai Tseng, Chun-Kuang Lin, Hsueh-Wei Chang, Yu-Hsuan Wu, Feng-Lin Yen, Fang-Rong Chang, Wei-Chun Chen, Chi-Chen Yeh, Jin-Ching Lee* (2014)Aqueous Extract of Gracilaria tenuistipitata Suppresses LPS-Induced NF-kB and MAPK Activation in RAW 264.7 and Rat Peritoneal Macrophages and Exerts Hepatoprotective Effects on Carbon Tetrachloride- Treated Rat. PLoS ONE 9(1): e86557. doi: 10.1371/journal.pone.0086557. (IF=3.7; 7/56 =12.5%)
- Chun-Chieh Lin, Peiju Tsai, Hung-Yu Sun, Mei-Chi Hsu, Jin-Ching Lee , I-Chin Wu, Chiung-Wen Tsao, Ting-Tsung Chang, Kung-Chia Young*.(2014)Apolipoprotein J, a glucose up-regulated molecular chaperone stabilizes core and NS5A to promote infectious hepatitis C virus virion production. Journal of Hepatology. 61(5), 984-993
- Yuan-Bin Cheng,Yi-Ting Chien,Jin-Ching Lee , Chin-Kai Tseng, Hui-ChunWang, I-Wen Lo, Yi-Hung Wu,Sheng-Yang Wang,Yang-Chang Wu,and Fang-Rong Chang. (2014)Limonoids from the Seeds of Swietenia macrophylla with Inhibitory Activity against Dengue Virus 2. Journal of Natural of Products.95. 2155-2165. (IF=3.947; 21/199 =10.5%)
- Peng-Yeh Lai, Chia-Tse Hsu, Shao-Hung Wang, Jin-Ching Lee, Min-Jen Tseng, Jaulang Hwang, Wen-Tsai Ji and Hau-Ren Chen. (2014). Production of a neutralizing antibody against envelope protein of dengue virus 2 type 2 using linear array epitope (LAE) technique. Journal of General Virology. 95, 2155-2165 I.F.=3.127; 44/160 (27.5%)
- I-Ling Lin, Han-Lin Chou, Jin-Ching Lee , Feng-Wei Chen, Yao Fong, Wei-Chiao Chang, Hurng Wern Huang, Chang-Yi Wu, Wen-Tsan Chang, Hui-Min David Wang and Chien-Chih Chiu* (2014)The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB. Cancer Cell International. 14:1-7
2013:
- Wei-Chun Chen, Sheng-Yang Wang, Chien-Chih Chiu, Chin-Kai Tseng, Chun-Kuang Lin, Hui-Chun Wang and Jin-Ching Lee* . (2013)Lucidone Suppresses Hepatitis C Virus Replication by Nrf2-Mediated Heme Oxygenase-1 Induction. Antimicrobial Agents and Chemotherapy.57(3):1180-1191. (IF=4.5; 31/260 =11.9%)
- Kuan-Jen Chen., Chin-Kai Tseng, Fang-Rong Chang, Jin-Iong Yang, Chi-Chen Yeh, Wei-Chun Chen, Shou-Fang Wu, Hsueh-Wei Chang, Jin-Ching Lee* . (2013)Aqueous Extract of the Edible Gracilaria tenuistipitata Inhibits Hepatitis C Viral Replication via Cyclooxygenase- 2 Suppression and Reduces Virus-Induced Inflammation. PLoS ONE. 8(2): e57704. doi:10.1371/journal.pone.0057704. (IF=3.7; 7/56 =12.5%)
- Ying-Ting Lin, Yu-Hsuan Wu, Chin-Kai Tseng, Chun-Kuang Lin, Wei-Chun Chen, Yao-Chin Hsu, Jin-Ching Lee* . (2013)Green Tea Phenolic Epicatechins Inhibit Hepatitis C Virus Replication via Cycloxygenase-2 and Attenuate Virus-Induced Inflammation. PLoS ONE. 8(1): e54466. doi:10.1371/journal.pone.0054466. (IF=3.7; 7/56 =12.5%)
- Huang-Kai Peng, Wei-Chun Chen, Ying-Ting Lin, Chin-Kai Tseng, Shiang-Yu Yang, Cherng-Chyi Tzeng, Jin-Ching Lee* , Shyh-Chyun Yang*. (2013) Anti-hepatitis C virus RdRp activity and replication of novel anilinobenzothiazole derivatives. Antiviral Research 100: 269-275. (IF=3.92; 45/260 =17.3%)
- Hung-Yu Sun, Chun-Chieh Lin, Jin-Ching Lee , Shainn-Wei Wang, Pin-Nan Cheng, I-Chin Wu, Ting-Tsung Chang, Ming-Derg Lai, Dar-Bin Shieh, Kung-Chia Young. (2013) Very low-density lipoprotein/lipo-viro particles reverse lipoprotein lipase-mediated inhibition of hepatitis C virus infection via apolipoprotein C-III. Gut. 62:1193–1203. (IF=10.732; 3/74 =4%)
- Huang-Kai Peng, Wei-Chun Chen, Jin-Ching Lee , Shiang-Yu Yang, Cherng-Chyi Tzeng, Ying-Ting Lin and Shyh-Chyun Yang. (2013)Novel anilinocoumarin derivatives as agents against hepatitis C virus by the induction of IFN-mediated antiviral responses. Organic & Biomolecular Chemistry. 11, 1858-1866. (IF=3.568; 11/55 =20%)
- Jen-Yang Tang, Jin-Ching Lee , Ming-Feng Hou,Chun-LinWang, Chien-Chi Chen, Hurng-Wern Huang, and Hsueh-Wei Chang. (2013)Alternative Splicing for Diseases, Cancers, Drugs, and Databases.Scientific World Journal vol 2013, Article ID 703568, 8 pages, doi: 10.1155/2013/703568. (IF=1.73; 13/56 =23.2%)
- Jin-Ching Lee , Ming-Feng Hou, Hurng-Wern Huang, Fang-Rong Chang, Chi-Chen Yeh, Jen-Yang Tang and Hsueh-Wei Chang. (2013)Marine algal natural products with anti-oxidative, anti-inflammatory, and anti-cancer properties. Cancer Cell International. 13:55.(IF=2.09; 121/196 =61.7%)
- Guan-Ting Liu, Chi-Shin Hwang, Chia-Hung Hsieh, Chih-Hao Lu, Sunny Li-Yun Chang, Jin-Ching Lee , Chien-Fu Huang, and Hao-Teng Chang. (2013) Eosinophil-Derived Neurotoxin Is Elevated in Patients with Amyotrophic Lateral Sclerosis. Mediators of Inflammation. ID 421389, 7 pages. doi: 10.1155/2013/421389. (IF=3.882; 39/135 =28.8%)
2012:
- Shou-Fang Wu, Chun-kuang Lin, Yen-Shu Chuang, Fang-Rong Chang, Chin-Kai Tseng, Yang-Chang Wu, and Jin-Ching Lee* (2012) Anti-Hepatitis C Virus Activity of 3-hydroxy caruilignan C from Swietenia macrophylla Stems. Journal of Viral Hepatitis 19, 364-370(IF=3.502; 19/72 =26%)
- Huang-Kai Peng, Chun-Kuang Lin, Chin-Kai Tseng, Cherng-Chyi Tzeng, Jin-Ching Lee* , and Shyh-Chyun Yang*. (2012) Synthesis and anti-HCV activity evaluation of anilinoquinoline derivatives. Bioorganic and Medicinal Chemistry Letters .22, 1107-1110. *corresponding author (IF=2.66; 19/56 =33.9%)
- Daniel B. Nichols, Guy Fournet, K.R. Gurukumar, Amartya Basu, Jin-Ching Lee , Naoya Sakamoto, Frank Kozielski, Ira Musmuca, Benoît Joseph, Rino Ragno, Neerja Kaushik-Basu. (2012). Inhibition of hepatitis C virus NS5B polymerase by S-trityl-L-cystein derivatives. European Journal of Medicinal Chemistry. 1-9.(IF=3.499; 13/59 =22%)
- Andriy G. Golub, K.R. Gurukumar, Amartya Basu, Volodymyr G. Bdzhola, Yaroslav Bilokin, Sergiy M. Yarmoluk, Jin-Ching Lee , Tanaji T. Talele, Daniel B. Nichols, Neerja Kaushik-Basu. (2012)Discovery of new scaffolds for rational design of HCV NS5B polymerase inhibitors. European Journal of Medicinal Chemistry. 58: 258-264. (IF=3.499; 13/59 =22%)
- Chi-Chen Yeh, Jing-Iong Yang, Jin-Ching Lee , Chao-Neng Tseng, Ya-Ching Chan, You-Cheng Hseu, Jen-Yang Tang, Li-Yeh Chuang, Hurng-Wern Huang, Fang-Rong Chang* and Hsueh-Wei Chang*. (2012)Anti-proliferative effect of methanolic extract of Gracilaria tenuistipitata on oral cancer cells involves apoptosis, DNA damage, and oxidativestress. BMC Complementary & Alternative Medicine. 12:142. (IF=2.082; 7/21 =33.3%)
- Jing-iong Yang, Chi-chen Yeh, Jin-Ching Lee , Szu-cheng Yi, Chao-neng Tseng, Hsueh-wei Chang , Jing-iong Yang, Chi-chen Yeh, Jin-ching Lee, Szu-cheng Yi, Chao-neng Tseng, Hsueh-wei Chang. (2012)Aqueous Extracts of the Edible Gracilaria tenuistipitata Are Protective Against H2O2-Induced DNA Damage, Growth Inhibition, and Cell Cycle Arrest. Molecules. 17, 7241-7254. (IF=2.428; 23/55 =41.8%)
2011:
- Chin-kai Tseng, Kuan-Jen Chen, Chun-Kuang Lin, Shih-hsien Hsu, Jin-Ching Lee * (2011) An in vitro Coupled Transcription/Translation Reporter System for Hepatitis C Virus RNA-dependent RNA Polymerase. Analytical Biochemistry 418, 50-57. (IF=3.236; 16/73 =21.9%)
- Jin-Ching Lee,* Chen WC, Wu SF, Tseng CK, Chiou CY, Chang FR, Hsu SH, Wu YC.(2011). Anti-hepatitis C virus activity of Acacia confusa extract via suppressing cyclooxygenase-2. Antiviral Research. 89, 35-42(IF=4.439; 34/252 =13.4%)
- Jin-Ching Lee,* C.-K. Tseng, S.-F. Wu, F.-R. Chang, C.-C. Chiu and Y.-C. Wu. (2011).San-Huang-Xie-Xin-Tang extract suppresses hepatitis C virus replication and virus-induced cyclooxygenase-2 expression. Journal of Viral Hepatitis 18, e315-e324 (IF=3.502; 19/72 =26%)
- Ying-Ting Lin, Kuo-Jung Huang, Chin-Kai Tseng, Kuan-Jen Chen, Hui-Min Wang, and Jin-Ching Lee* (2011). Efficient In Silico Assay of Inhibitors of Hepatitis C Virus RNA-Dpendent RNA Polymerase by Structure-Based Virtual Screening and In Vitro Evaluation. ASSAY and Drug Development Technologies.(DOI: 10.1089/adt.2010.0341)(IF=2.713; 25/67 =37%)
研討會論文
- Jin-Ching Lee*, Wei-Chun Chen.Hepatitis C virus NS5A protein up regulates cyclooxygenase-2 expression via IL-8-mediated ERK/JNK MAPK pathway. 12th Annual Meeting of Japanese Society of Medical Oncology. (2014, July 17-19). Fukuoka, Japan.
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Jin-Ching Lee*, Chun-Kuang Lin, Chin-Kai Tseng. Identification of prostasin serine protease against hepatitis C virus replication. 12th Human proteome Organization World Congress. (2013, Sept. 14-18 ). Yokohama, Japan
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Jin-Ching Lee*, Hsueh-Wei Chang, Kuan-Jen Chen, Chin-Kai Tseng, Fang-Rong Chang, Jin-Iong Yang. Aqueous extract of the seaweed Gracilaria tenuistipitata inhibits hepatitis C viral replication via cyclooxygenase-2 suppression and attenuates virus-induced inflammation. The 9th Asia-Pacific marine Biotechnology Conference. (2012,July 13-16 ). Kochi, Japan.
- Jin-Ching Lee* Anti-Hepatitis C Virus Activity of Acacia confusae Extract via Suppressing the Cyclooxygenase. 2BIT's 1st World Congress of Virus and Infections (WCVI) which will be held during July 31~August 3, 2010 in Busan, Republic of Korea.
- Chin-kai Tseng, Chun-Kuang Lin, Jin-Ching Lee*. A rapid reporter assay for drug screening of anti-hepatitis C virus RNA-dependent RNA polymerase. IBC’s 4th Annual Drug Discovery & Development 2009. JAPAN conference
- Wei-Chun Chen, Chin-kai Tseng, Yu-Fen Chen, Jin-Ching Lee*. Establishment of hepatitis C virus-pseudotyped baculovirus for screening inhibitors against viral entry .BIT's 1st Annual World Summit of Antivirals (WSA-2008). July 20-22, 2008 in Kunming.
- Chin-kai, Tseng , Yang-Chang Wu , Chun-Kuang Lin , Fang-Rong Chang & Jin-Ching Lee*. Inhibition of hepatitis c virus replication by catechin extracted from Chinese and Herbal Medicine. 2007 10th Southeast Asian Western Pacific Regional Meeting of Pharmacologists and the 41st Annual Scientific Meeting of ASCEPT.
專利：
1. 用於抑制癌細胞生長、誘導癌細胞細胞凋亡及/或造成DNA傷害之藥物組成物，以及紅藻龍需菜醇類萃取物的用途 （中華民國 I458486; 2014.11.01~2033.02.26）
2. 用於減少自由機誘導之細胞之DNA損傷的試劑、藥物或保健食品、與皮膚保養或化妝品，以及紅藻龍需菜萃取物的用途 （中華民國 I492766; 201507.21~2031.01.20）
3. 2-苯胺基-6-甲基苯并噻唑衍生物及其製備方法與用途（中華民國 I543973; 201608.01）
4. 五味子甲素之用途（中華民國 I544918; 201608.11）
5. 五味子素之用途（中華民國 I568437; 201702.01）
6. 赤芝酮之用途（中華民國 I569796; 201702.11）
7. 酪梨萃取物、avocadenol B 及(2R，4R)-1，2，4-三羥基十七碳-16-炔的用途，以及包含酪梨萃取物之保健食品（中華民國 I605822; 201711.21）
實驗室成員
實驗室成員
Lab1024 member
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